Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture of Phaeoacremonium Species

Grapevine ( L.) can be affected by many different biotic agents, including tracheomycotic fungi such as and , which are the main causal agent of Esca and Petri diseases. Both fungi produce phytotoxic naphthalenone polyketides, namely scytalone and isosclerone, that are related to symptom development...

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Published in:Journal of fungi (Basel) 2022-01, Vol.8 (1), p.55
Main Authors: Reveglia, Pierluigi, Raimondo, Maria Luisa, Masi, Marco, Cimmino, Alessio, Nuzzo, Genoveffa, Corso, Gaetano, Fontana, Angelo, Carlucci, Antonia, Evidente, Antonio
Format: Article
Language:English
Subjects:
Bioassays
Chromatography
Disease
Esca complex disease
Fungi
Investigations
isosclerone
LC-MS/MS
Metabolism
Metabolites
Metabolomics
Pathogens
Phaeoacremonium
Phaeoacremonium spp
Phytotoxicity
Polyketides
Scytalone
Secondary metabolites
Species
Virulence
Wineries & vineyards
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Summary:Grapevine ( L.) can be affected by many different biotic agents, including tracheomycotic fungi such as and , which are the main causal agent of Esca and Petri diseases. Both fungi produce phytotoxic naphthalenone polyketides, namely scytalone and isosclerone, that are related to symptom development. The main objective of this study was to investigate the secondary metabolites produced by three species and to assess their phytotoxicity by in vitro bioassay. To this aim, untargeted and targeted LC-MS/MS-based metabolomics were performed. High resolution mass spectrometer UHPLC-Orbitrap was used for the untargeted profiling and dereplication of secondary metabolites. A sensitive multi reaction monitoring (MRM) method for the absolute quantification of scytalone and isosclerone was developed on a UPLC-QTrap. Different isolates of , and were grown in vitro and the culture filtrates and organic extracts were assayed for phytotoxicity. The toxic effects varied within and among fungal isolates. Isosclerone and scytalone were dereplicated by matching retention times and HRMS and MS/MS data with pure standards. The amount of scytalone and isosclerone differed within and among fungal species. To our best knowledge, this is the first study that applies an approach of LC-MS/MS-based metabolomics to investigate differences in the metabolic composition of organic extracts of species culture filtrates.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof8010055