Gene expression profile for different susceptibilities to sound stimulation: a comparative study on brainstems between two inbred laboratory mouse strains

• Published in: BMC genomics 2022-11, Vol.23 (1), p.783-783, Article 783
• Main Authors: Zhu, Lina, Chen, Deng, Lin, Xin, Liu, Ling
• Format: Article
• Language: English
• Subjects: AGSz; Analysis; Animal experimentation; Animals; Auditory stimuli; Bioinformatics; Brain Stem; Comparative studies; Convulsions & seizures; Disease susceptibility; DNA microarrays; Epigenetics; Epilepsy; Evaluation; Gene expression; Genetic aspects; Genomics; Inbreeding; Laboratories; Long noncoding RNAs; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Mutation; Non-coding RNA; RNA, Long Noncoding - genetics; RNA, Messenger - genetics; S-IRA; Seizures; Signal transduction; Signaling; Sound; Stimulation; SUDEP model; Susceptibility; Transcriptome; Transforming Growth Factor beta
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-022-09016-3

DBA/1 mice have a higher susceptibility to generalized audiogenic seizures (AGSz) and seizure-induced respiratory arrest (S-IRA) than C57/BL6 mice. The gene expression profile might be potentially related to this difference. This study aimed to investigate the susceptibility difference in AGSz and S-IRA between DBA/1 and C57BL/6 mice by profiling long noncoding RNAs (lncRNAs) and mRNA expression. We compared lncRNAs and mRNAs from the brainstem of the two strains with Arraystar Mouse lncRNA Microarray V3.0 (Arraystar, Rockville, MD). Gene Ontology (GO) and pathway analyses were performed to determine the potentially related biological functions and pathways based on differentially expressed mRNAs. qRT-PCR was carried out to validate the results. A total of 897 lncRNAs and 438 mRNAs were differentially expressed (fold change ≥2, P