Evaluation of the expression of P-glycoprotein in propoxur-resistant Caco-2 cells

There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. T...

Full description

Saved in:
Bibliographic Details
Published in:Acta medica Iranica 2014, Vol.52 (10), p.728-733
Main Authors: Yazdian, Shabnam, Fahham, Najmeh, Ghahremani, Mohammad Hossein, Afzali, Monireh, Farsandaj, Narges, Vatankhah, Melody, Ostad, Seyed Nasser
Format: Article
Language:English
Subjects:
ATP-Binding Cassette, Sub-Family B, Member 1 - genetics
Caco-2 Cells
Cell Membrane - metabolism
Drug Resistance
Humans
P-glycoprotein
Propoxur
Propoxur - pharmacology
Resistant
Toxicity
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells.  Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells. This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.
ISSN:0044-6025
1735-9694