Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells

Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hypertrophy wa...

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Bibliographic Details
Published in:Scientific reports 2021-01, Vol.11 (1), p.1515-1515, Article 1515
Main Authors: Kawaguchi, Takahisa, Hasegawa, Kazuhiro, Yasuda, Itaru, Muraoka, Hirokazu, Umino, Hiroyuki, Tokuyama, Hirobumi, Hashiguchi, Akinori, Wakino, Shu, Itoh, Hiroshi
Format: Article
Language:English
Subjects:
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Adult
Aged
Animals
Apoptosis
Bowman Capsule - metabolism
Cell cycle
Cell Differentiation
Cells, Cultured
Diabetes
Diabetes mellitus
Diabetes Mellitus - metabolism
Diabetes Mellitus - physiopathology
Diabetes Mellitus, Experimental - metabolism
Diabetic Nephropathies - metabolism
Diabetic nephropathy
Disease Models, Animal
Epithelial cells
Epithelial Cells - metabolism
Female
Flow cytometry
Glomerulus
Humanities and Social Sciences
Humans
Hypertrophy
Hypertrophy - physiopathology
Kidney Glomerulus - pathology
Male
Mice
Mice, Obese
Middle Aged
multidisciplinary
Nephropathy
Podocytes - metabolism
Podocytes - pathology
Podocytes - physiology
S phase
Science
Science (multidisciplinary)
Vacuoles
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Summary:Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hypertrophy was significantly more prominent in diabetic mice than in nondiabetic mice, and this was evident even at the early stage. Additionally, the number of vacuoles in PECs was markedly increased in diabetic mice, suggesting the presence of cellular injury in PECs in DN. Although rare, binuclear cells were observed in mice with early diabetes. In cultured PECs, a high glucose condition, compared with normal glucose condition, induced cellular hypertrophy and apoptosis. Flow cytometry showed that some PECs in the G0 phase reentered the cell cycle but got arrested in the S phase. Finally, in human diabetic subjects, hypertrophy and vacuolization were observed in the PECs. Our data showed that PECs undergo substantial changes in DN and may participate in rearrangement for differentiation into podocytes.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-81027-8