Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

We present the first cytogenetic data for and with the aim of (1) investigating evolutionary events within and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with si...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-06, Vol.20 (12), p.2944
Main Authors: Sassi, Francisco de Menezes Cavalcante, Oliveira, Ezequiel Aguiar de, Bertollo, Luiz Antonio Carlos, Nirchio, Mauro, Hatanaka, Terumi, Marinho, Manoela Maria Ferreira, Moreira-Filho, Orlando, Aroutiounian, Rouben, Liehr, Thomas, Al-Rikabi, Ahmed B H, Cioffi, Marcelo de Bello
Format: Article
Language:English
Subjects:
Biological evolution
Chromosomes
comparative genomic hybridization
Cytogenetics
Deoxyribonucleic acid
DNA
Evolution
Experiments
Females
Fish
Hybridization
karyotype evolution
Karyotypes
Lebiasinidae
Males
Phylogenetics
Ribosomal DNA
Sex chromosomes
Sexual dimorphism
Taxonomy
whole chromosome painting
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Summary:We present the first cytogenetic data for and with the aim of (1) investigating evolutionary events within and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in is absent in . Accordingly, shows the ribosomal DNA sites as the only GC-rich (CMA ) regions, while shows evidence of a clear intercalated CMA banding pattern. In addition, the multiple 5S and 18S rDNA sites in contrast with single sites present in . Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA , and (CGG)n band was found only to occur in females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the species examined and share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20122944