Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts

Zika virus (ZIKV) infection at the maternal–placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into...

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Bibliographic Details
Published in:Scientific reports 2022-05, Vol.12 (1), p.7348-7348, Article 7348
Main Authors: Block, Lindsey N., Schmidt, Jenna Kropp, Keuler, Nicholas S., McKeon, Megan C., Bowman, Brittany D., Wiepz, Gregory J., Golos, Thaddeus G.
Format: Article
Language:English
Subjects:
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Animals
Cell differentiation
Extracellular vesicles
Extracellular Vesicles - genetics
Female
Fetuses
Gene Expression
Gestation
Humanities and Social Sciences
Humans
Infections
Interferon
Macaca mulatta
miRNA
multidisciplinary
Placenta
Placenta - metabolism
Pregnancy
Pregnancy Complications, Infectious
Science
Science (multidisciplinary)
Stem cells
Transcriptomes
Trophoblasts
Trophoblasts - metabolism
Vector-borne diseases
Zika virus
Zika Virus - physiology
Zika Virus Infection
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Summary:Zika virus (ZIKV) infection at the maternal–placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into early gestation syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT). TSCs and STs, but not EVTs, were highly permissive to productive infection with ZIKV strain DAK AR 41524. The impact of ZIKV on the cellular transcriptome showed that infection of TSCs and STs increased expression of immune related genes, including those involved in type I and type III interferon responses. ZIKV exposure altered extracellular vesicle (EV) mRNA, miRNA and protein cargo, including ZIKV proteins, regardless of productive infection. These findings suggest that early gestation macaque TSCs and STs are permissive to ZIKV infection, and that EV analysis may provide a foundation for identifying non-invasive biomarkers of placental infection in a highly translational model.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-11275-9