Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue

Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovaria...

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Bibliographic Details
Published in:Scientific reports 2017-08, Vol.7 (1), p.8203-11, Article 8203
Main Authors: Man, Limor, Park, Laura, Bodine, Richard, Ginsberg, Michael, Zaninovic, Nikica, Man, Omar Alexander, Schattman, Glenn, Rosenwaks, Zev, James, Daylon
Format: Article
Language:English
Subjects:
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Angiogenesis
Cryopreservation
Endothelial cells
Endothelium
Fertility
Follicles
Humanities and Social Sciences
Hypoxia
Infertility
Ischemia
multidisciplinary
Ovaries
Paracrine signalling
Patients
Perfusion
Reperfusion
Reproductive technologies
Science
Science (multidisciplinary)
Tissues
Transplantation
Transplants & implants
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Summary:Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovarian tissue that is co-transplanted with exogenous endothelial cells (ExEC). ExECs were capable of forming functionally perfused vessels at the host/graft interface and increased both viability and follicular volume in ExEC-assisted grafts with resumption of antral follicle development in long-term grafts. ExECs that were engineered to constitutively express anti-mullerian hormone (AMH) induced a greater proportion of quiescent primordial follicles than control ExECs, indicating suppression of premature mobilization that has been noted in the context of ovarian tissue transplantation. These findings present a cell-based strategy that combines accelerated perfusion with direct paracrine delivery of a bioactive payload to transplanted ovarian tissue.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-08491-z