Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid

Nowadays the development of novel biomaterials which would promote tissue lesions regeneration is one of the crucial tasks of regenerative medicine. In the current research we developed a biological active membrane composed of decellularized, lyophilized human amniotic membrane in combination with g...

Full description

Saved in:
Bibliographic Details
Published in:Journal of education, health and sport health and sport, 2019-11, Vol.9 (11), p.410-418
Main Authors: Vyshnevska, H. O., Kakabadze, Z. Sh, Schnayder, S. A., Tiron, O. I., Dzygal, O. F.
Format: Article
Language:English
Subjects:
amniotic membrane
growth factors
hyaluronic acid
morphology
scanning electron microscopy
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nowadays the development of novel biomaterials which would promote tissue lesions regeneration is one of the crucial tasks of regenerative medicine. In the current research we developed a biological active membrane composed of decellularized, lyophilized human amniotic membrane in combination with growth factors of platelet-rich plasma (PRP) and hyaluronic acid. On the basis of histological examination, scanning electron microscopic examination and DNA content analyses it was drawn a conclusion that Sodium Dodecyl Sulfate (SDS) (Sigma) solution and 1% Triton X-100 solution completely remove all existing cells of placenta and chorionic-amniotic membrane without impairment of their structure. After the processes of decellularization and lyophilization the DNA content was less than 2%. The PRP growth factors as the components biological matrix provide faster regeneration. The latter are fixed on the surface of cellular structure of decellularized, lyophilized human amniotic membrane by the reservoir of gelatinous hyaluronic acid
ISSN:2391-8306
2391-8306
DOI:10.12775/JEHS.2019.09.11.038