Sequencing and analysis of the complete mitochondrial genomes of Toona sinensis and Toona ciliata reveal evolutionary features of Toona

• Published in: BMC genomics 2023-02, Vol.24 (1), p.58-58, Article 58
• Main Authors: Li, Youli, Gu, Min, Liu, Xuanzhe, Lin, Jianna, Jiang, Huier, Song, Huiyun, Xiao, Xingcui, Zhou, Wei
• Format: Article
• Language: English
• Subjects: Annotations; Botanical research; Chloroplasts; Codons; DNA sequencing; Evolution; Gene mapping; Genes; Genetic engineering; Genome, Mitochondrial; Genomes; Genomics; High-throughput sequencing; Meliaceae - genetics; Mitochondria; Mitochondria genome; Mitochondrial DNA; Nucleotide sequencing; Phylogenetic relationship; Phylogenetics; Phylogeny; Plant Breeding; Proteins; Software; Structure; Structure-function relationships; Taxonomy; Toona; Toona - genetics; Toona ciliata; Toona ciliate; Toona sinensis; Transfer RNA; Trees; tRNA
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-023-09150-6

Toona is a critical genus in the Meliaceae, and the plants of this group are an asset for both restorative and restorative purposes, the most flexible of which are Toona sinensis and Toona ciliata. To concentrate on the advancement of mitochondrial(Mt) genome variety in T.sinensis and T.ciliata, the Mt genomes of the two species were sequenced in high throughput independently, after de novo assembly and annotation to construct a Mt genome map for comparison in genome structure. Find their repetitive sequences and analyze them in comparison with the chloroplast genome, along with Maximum-likelihood(ML) phylogenetic analysis with 16 other relatives. (1) T. sinensis and T.ciliata are both circular structures with lengths of 683482 bp and 68300 bp, respectively. They share a high degree of similarity in encoding genes and have AT preferences. All of them have the largest Phe concentration and are the most frequently used codons. (2) Both of their Mt genome are highly preserved in terms of structural and functional genes, while the main variability is reflected in the length of tRNA, the number of genes, and the value of RSCU. (3) T. siniensis and T. ciliata were detected to have 94 and 87 SSRs, respectively, of which mononucleotides accounted for the absolute proportion. Besides, the vast majority of their SSRs were found to be poly-A or poly-T. (4)10 and 11 migrating fragments were identified in the comparison with the chloroplast genome, respectively. (5) In the ML evolutionary tree, T.sinensis and T.ciliata clustered individually into a small branch with 100% support, reflecting two species of Toona are very similarly related to each other. This research provides a basis for the exploitation of T.sinensis and T.ciliata in terms of medicinal, edible, and timber resources to avoid confusion; at the same time, it can explore the evolutionary relationship between the Toona and related species, which does not only have an important practical value, but also provides a theoretical basis for future hybrid breeding of forest trees, molecular markers, and evolutionary aspects of plants, which has great scientific significance.