Comparative Study Of Genetic Diversity, Virulence Genotype, Biofilm Formation And Antimicrobial Resistance Of Uropathogenic Escherichia coli (UPEC) Isolated From Nosocomial And Community Acquired Urinary Tract Infections

is a Gram-negative opportunistic human pathogen, which has aroused considerable medical interest for being involved in cases of urinary tract infection. Characterize the isolated both in the hospital and in the community. A total of 200 isolated in urine samples from hospital and community were eval...

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Bibliographic Details
Published in:Infection and drug resistance 2019-11, Vol.12, p.3595-3606
Main Authors: De Souza, Gabrielle Messias, Neto, Estevan Rodrigues Dos Santos, da Silva, Alaor Martins, Iacia, Maria Vitoria Minzoni de Souza, Rodrigues, Marcus Vinícius Pimenta, Cataneli Pereira, Valéria, Winkelstroter, Lizziane Kretli
Format: Article
Language:English
Subjects:
adhesion
Beta lactamases
Biodiversity
Comparative analysis
Comparative literature
control
esbl
Genes
Genetic aspects
Health aspects
Microbial drug resistance
Microorganisms
Original Research
Phylogeny
Polymerase chain reaction
Urinary tract infections
uti
Virulence (Microbiology)
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Summary:is a Gram-negative opportunistic human pathogen, which has aroused considerable medical interest for being involved in cases of urinary tract infection. Characterize the isolated both in the hospital and in the community. A total of 200 isolated in urine samples from hospital and community were evaluated in biofilm formation assay and hydrophobicity MATS method. Antimicrobial susceptibility was performed through agar-diffusion technique. Virulence and ESBL production genes were observed through the polymerase chain reaction amplification of , and The phylogenetic classification was based on the pattern and and the region TspE4.C2 by PCR Multiplex. A higher frequency of non-adherent or poorly adherent isolates was observed in the community group. Approximately 85% of the community isolates were distributed in the highest hydrophilicity group (p0.05). About 14% of the hospital isolates were positive in the ESBL phenotypic detection test (p>0.05). Among the samples, 95% presented ESBL-encoding genes. The predominant phylogenetic group was B2 (78%). Community isolates showed a higher prevalence of virulence genes , and when compared to hospital samples. These data confirm the worldwide trend that isolates in the community present sometimes higher levels of virulence and antimicrobial resistance.
ISSN:1178-6973
1178-6973
DOI:10.2147/IDR.S228612