Genetic and Transgenic Reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis

Abstract Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagent...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2017-04, Vol.7 (4), p.1339-1347
Main Authors: Stern, David L, Crocker, Justin, Ding, Yun, Frankel, Nicolas, Kappes, Gretchen, Kim, Elizabeth, Kuzmickas, Ryan, Lemire, Andrew, Mast, Joshua D, Picard, Serge
Format: Article
Language:English
Subjects:
Alleles
Animals
Animals, Genetically Modified
DNA Transposable Elements - genetics
Drosophila
Drosophila - genetics
Drosophila simulans - genetics
evolution
Eye - metabolism
Gene Expression Regulation
genetics
Genomics
Green Fluorescent Proteins - metabolism
Investigations
Mutagenesis, Insertional - genetics
speciation
Species Specificity
Transgenes
transgenics
ϕC31 integrase
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Summary:Abstract Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a ϕC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies.
ISSN:2160-1836
2160-1836
DOI:10.1534/g3.116.038885