Platelet aggregation response to cyclooxygenase inhibition and thromboxane receptor antagonism using impedance aggregometry: A pilot study

Impedance aggregometry is an alternative to light transmission aggregometry that allows analysis of platelet function in whole blood samples. We hypothesized (1) impedance aggregometry would produce repeatable results, (2) inhibition of cyclooxygenase with aspirin would attenuate aggregation respons...

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Bibliographic Details
Published in:Physiological reports 2024-08, Vol.12 (16), p.e70002-n/a
Main Authors: Williams, Auni C., Fisher, Kat G., Alexander, Lacy M., Kenney, W. Larry
Format: Article
Language:English
Subjects:
Adult
Arachidonic acid
Arachidonic Acid - pharmacology
Aspirin
Aspirin - pharmacology
Blood diseases
Blood platelets
Blood Platelets - drug effects
Blood Platelets - metabolism
Cardiovascular disease
Collagen
Collagen - pharmacology
COX inhibition
Cyclooxygenase Inhibitors - pharmacology
Electric Impedance
Female
Humans
impedance aggregometry
Male
Middle Aged
Naphthalenes - pharmacology
Pilot Projects
Platelet aggregation
Platelet Aggregation - drug effects
Platelet Aggregation Inhibitors - pharmacology
Platelet Function Tests - methods
Propionates - pharmacology
Prostaglandin endoperoxide synthase
Receptors, Thromboxane - antagonists & inhibitors
Receptors, Thromboxane - metabolism
Regular Manuscript
Short Report
Software
thromboxane receptor inhibition
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Summary:Impedance aggregometry is an alternative to light transmission aggregometry that allows analysis of platelet function in whole blood samples. We hypothesized (1) impedance aggregometry would produce repeatable results, (2) inhibition of cyclooxygenase with aspirin would attenuate aggregation responses to collagen and abolish the aggregation response to arachidonic acid (AA), and (3) thromboxane receptor antagonism (terutroban) would attenuate the aggregation response to AA. Venous blood was obtained from 11 participants three times separated by at least 2 weeks. One sample followed 7‐day‐aspirin intervention (81 mg once daily; ASA), the others no intervention (control). Aggregation was induced using 1 μg/mL collagen ([col 1]), 5 μg/mL collagen ([col 5]), and 50 mM AA via impedance aggregometry to determine total aggregation (AUC) analyzed for intra‐test repeatability, inter‐test repeatability, intervention (ASA or control), and incubation (saline or terutroban). [col 1] showed high intra‐test (p ≤ 0.03 visit 1 and 2) and inter‐test repeatability (p 
ISSN:2051-817X
2051-817X
DOI:10.14814/phy2.70002