Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages

In inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We est...

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Bibliographic Details
Published in:Mediators of Inflammation 2006-01, Vol.2006 (6), p.220-227
Main Authors: JALONEN, Ulla, NIEMINEN, Riina, VUOLTEENAHO, Katriina, KANKAANRANTA, Hannu, MOILANEN, Eeva
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cytokines
Gene expression
General aspects
Interleukins
Macrophages
Medical sciences
Research Communication
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Summary:In inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We established a TTP knock-down cell line by expressing shRNA against TTP (shTTP cell line). A cytokine antibody array was used to measure cytokine production in macrophages exposed to lipopolysaccharide (LPS). Cytokines IL-6, IL-12, TNF- α , and MIP-2 (a homologue to human IL-8) were expressed at higher levels whereas MIP-3 α was produced at lower levels in LPS-treated shTTP cells than in control cells suggesting that the expression of these cytokines is regulated by TTP. The present data provide IL-12, MIP-2, and MIP-3 α as novel inflammatory cytokine targets for TTP-mediated mRNA decay and stress the role of TTP in the regulation of the inflammatory process.
ISSN:0962-9351
1466-1861
DOI:10.1155/MI/2006/40691