Applying infectious clones and untargeted metabolite profiling to characterize citrus tristeza virus-induced stem pitting in citrus

• Published in: Scientific reports 2024-11, Vol.14 (1), p.28490-15, Article 28490
• Main Authors: Aldrich, D. J., Taylor, M., Bester, R., El-Mohtar, C. A., Burger, J. T., Maree, H. J.
• Format: Article
• Language: English
• Subjects: 631/337; 631/449; 631/61; Citrus - virology; Citrus fruits; Cloning; Closterovirus; Closterovirus - genetics; Closterovirus - metabolism; Corrosion; Economic importance; Experiments; Fruits; Genomes; Genotype & phenotype; Genotypes; Glycosides; Humanities and Social Sciences; Infections; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; multidisciplinary; Open Reading Frames; Pathogenicity; Pathogens; Phenolic acids; Phenols; Plant Diseases - virology; Plant Stems - metabolism; Plant Stems - virology; Plant viruses; Science; Science (multidisciplinary); Scientific imaging; Statistical analysis; Stem pitting; Trends
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-79402-2

Citrus tristeza virus (CTV) causes economically important stem pitting in sensitive citrus types however the exact mechanisms of stem pitting development in citrus remain unclear. In this study, CTV infectious clones were used to study stem pitting induction in ‘Duncan’ grapefruit and ‘Mexican’ lime. A panel of open reading frame (ORF) replacement clones was generated focusing on the CTV ORFs implicated in stem pitting development and pathogenicity, namely p33, p18, p13 and p23. ORF replacements from severe- and mild-pitting CTV isolates were introduced into a mild-pitting infectious clone (genotype T36) to determine if stem pitting could be induced. A broad range of stem pitting outcomes were observed with ORF p18 (from isolate T3-KB) and ORF p23 (from isolate GFMS12-1.3) associated with enhanced stem pitting development. Metabolomic trends underlying the different stem pitting outcomes were further assessed by untargeted metabolite profiling. In each citrus host, the metabolite profiling identified statistically significant compounds that differed between stem pitting groups. These compounds were mainly phenolic acids and phenolic glycosides and are known to function as antioxidant and stress-signaling molecules. These metabolites can serve as targets for future time-course observations to potentially use mass spectrometry profiling to inform CTV management practices.