Antimicrobial properties of dental cements modified with zein-coated magnesium oxide nanoparticles

• Published in: Bioactive materials 2022-02, Vol.8, p.49-56
• Main Authors: Naguib, Ghada H., Nassar, Hani M., Hamed, Mohamed T.
• Format: Article
• Language: English
• Subjects: Antimicrobial biomaterials; Dental cement; Magnesium oxide nanoparticles; Nanocoatings
• ISSN: 2452-199X; 2452-199X
• DOI: 10.1016/j.bioactmat.2021.06.011

The aim of this study was to test the antimicrobial properties of dental cements modified with magnesium oxide (MgO) nanoparticles. Zein-modified MgO nanoparticles (zMgO) in concentrations (0.0, 0.3, 0.5, and 1.0%) were mixed with dental cements (Fuji II, Rely X Temp E, Ionoglass Cem, Es Temp NE, and System P link). Eight discs were fabricated from each zMgO-cement pair for a total of 32 specimens for each cement. Characterization of the dental cements incorporating zMgO was done by X-ray Diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM). The antimicrobial properties of the mixtures were tested using direct contact and agar diffusion assays against Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Data was analyzed using two-way analysis of variance and LSD post hoc test at 0.05 significance level. XRD spectra showed sharp peaks of zMgO indicating its high crystalline nature, while the amorphous dental cements with zMgO had broad peaks. FESEM analysis showed a uniform distribution of the zMgO nanoparticles in the cement. There were significant inhibition zone values associated with all concentrations of zMgO-cement mixtures tested compared to controls (p < 0.001) with a dose-response recorded only with Fuji II. Optical density values were significantly lower in zMgO groups compared to controls for all microorganisms. The effect was most prominent with Rely X against C. albicans and S. aureus. Dental cements containing zMgO showed significant antimicrobial properties that were dependent on the specific initial cement substrate. [Display omitted] •Antimicrobial nanoparticles (NPs) are widely used in dental materials to improve their biological properties.•Magnesium Oxide (MgO) NPs are novel antimicrobial agents.•Incorporation of MgO NPs in dental cements aids in minimizing bacterial colonization at the restoration margin.•Zein polymer facilitates the dispersion of MgO NPs and avoid its agglomeration.•Zein polymer effectively enhances the performance of MgO NPs.