Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure

• Published in: Canadian respiratory journal 2021-06, Vol.2021, p.6652297-12
• Main Authors: Gan, Qing, Tang, Donge, Yan, Qiang, Chen, Jiejing, Xu, Yong, Xue, Wen, Xiao, Lu, Zheng, Fengping, Xu, Huixuan, Fu, Yingyun, Dai, Yong
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Biological markers; Biomarkers; China; Chromatography; Chromatography, Liquid; Chronic obstructive pulmonary disease; Comparative analysis; Complications and side effects; Development and progression; Disease; Diseases; Ethylenediaminetetraacetic acid; Female; Females; Gene expression; Health aspects; Hospitals; Humans; Liquid chromatography; Lung diseases, Obstructive; Lysine; Lysine - blood; Male; Males; Mass spectrometry; Medical research; Medicine, Experimental; Outdoor air quality; Peptides; Physiological aspects; Post-translational modification; Proteins; Proteome - analysis; Pulmonary Disease, Chronic Obstructive - blood; Pulmonary Disease, Chronic Obstructive - complications; Respiratory failure; Respiratory insufficiency; Respiratory Insufficiency - blood; Respiratory Insufficiency - complications; Risk factors; Solvents; Sperm; Tandem Mass Spectrometry
• ISSN: 1198-2241; 1916-7245
• DOI: 10.1155/2021/6652297

Introduction. The modification of lysine crotonylation (Kcr) is another biological function of histone in addition to modification of lysine acetylation (Kac), which may play a specific regulatory role in diseases. Objectives. This study compared the expression levels of Kcr and proteome between patients with chronic obstructive pulmonary disease (COPD) combined with type II respiratory failure (RF) to study the relationship between Kcr, proteome, and COPD. Methods. We tested the Kcr and proteome of COPD combined with type II RF and normal control (NC) using croton acylation enrichment technology and liquid chromatography tandem mass spectrometry (LC-MS/MS) with high resolution. Results. We found that 32 sites of 23 proteins were upregulated and 914 sites of 295 proteins were downregulated. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG), protein domain, and Gene Ontology (GO) analysis on crotonylated protein. In proteomics research, we found that 190 proteins were upregulated and 151 proteins were downregulated. Among them, 90 proteins were both modified by differentially expressed crotonylation sites and differentially expressed in COPD combined with type II RF and NC. Conclusion. Differentially expressed crotonylation sites may be involved in the development of COPD combined with type II RF. 90 proteins modified by crotonylation and differentially expressed in COPD combined with type II RF can be used as markers for the study of the molecular pathogenesis of COPD combined with type II RF.