Characterization of Novel RHD Allele Variants and Their Implications for Routine Blood Group Diagnostics

• Published in: Biomedicines 2024-02, Vol.12 (2), p.456
• Main Authors: Matzhold, Eva M, Bemelmans, Maria, Polin, Helene, Körmöczi, Günther F, Schönbacher, Marlies, Wagner, Thomas
• Format: Article
• Language: English
• Subjects: Alleles; Annealing; Antibodies; Antigens; Automation; Bioinformatics; Blood & organ donations; Blood groups; D antigen; Epitopes; Fetuses; Flow cytometry; Genes; Genetic testing; Genotyping; Haplotypes; Hemolytic disease; Immunogenicity; Monoclonal antibodies; Mutation; partial D; Phenotypes; Proteins; Rh diagnostics; Rh system; RHD allele; Rhesus blood group; Stop codon; Thermal cycling; weak D
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines12020456

The Rh system, including the highly immunogenic D antigen, is one of the clinically most important blood group systems in transfusion medicine. Numerous alleles of the gene are associated with variant RhD phenotypes. In case of Rh incompatibility, some of them can induce hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. Thus, accurate blood group diagnostics are critical for safe transfusion therapy. We characterized phenotypes of four individuals revealing weakened D expression during routine pre-transfusion testing. Standard gel card matrix techniques with monoclonal and polyclonal anti-D antibodies were used for serological typing, complemented using D epitope and antigen density analysis. Genotyping employing PCR with sequence-specific primers, genomic and allele-specific Sanger sequencing and in silico protein analysis were performed. Four novel alleles associated with weak D or partial D phenotypes were identified. One of the mutations is predicted to disrupt the terminal stop codon and result in an elongated translation of the mutant D protein that phenotypically exhibits a loss of D epitopes. Furthermore, a hybrid gene formed with the homologue gene is described. The presented data enhances the understanding of the Rh system and may contribute to continued advances in blood group diagnostics.