A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

Arabinogalactan proteins (AGPs) are plant cell components found in the extracellular matrix that play crucial roles in fruit growth and development. AGPs demonstrate structural diversity due to the presence of a protein domain and an expanded carbohydrate moiety. Considering their molecular structur...

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Bibliographic Details
Published in:Plant methods 2023-11, Vol.19 (1), p.1-117, Article 117
Main Authors: Kutyrieva-Nowak, Nataliia, Leszczuk, Agata, Zdunek, Artur
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Antigenic determinants
Antigens
Arabinogalactan
Arabinogalactan proteins
Carbohydrates
Cell division
Cell wall
Enzyme-linked immunosorbent assay
Enzymes
Epitopes
Extracellular matrix
Fruit
Fruits
Glycobiology
Glycoproteins
Glycosylation
Identification and classification
Immunocytochemistry
Immunofluorescence
Labeling
Medical research
Microscopy
Molecular biology
Molecular structure
Molecular weight
Physiological aspects
Plant cells and tissues
Probes
Properties
Proteins
Qualitative analysis
Research methods
Review
Ripening
Spatial distribution
Temporal distribution
Tissue analysis
Western blotting
Western immunoblotting
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Summary:Arabinogalactan proteins (AGPs) are plant cell components found in the extracellular matrix that play crucial roles in fruit growth and development. AGPs demonstrate structural diversity due to the presence of a protein domain and an expanded carbohydrate moiety. Considering their molecular structure, the modification of glycosylation is a primary factor contributing to the functional variety of AGPs. This paper provides a concise overview of AGP research methods, emphasising their use in fruit tissue analysis and demonstrating the accessibility gaps in other tools used in such research (e.g. antibodies against protein moieties). It underscores fruit tissue as a valuable source of AGPs and emphasises the potential for future research to understand of AGP synthesis, degradation, and their roles in various physiological processes. Moreover, the application of advanced probes for AGP visualisation is a milestone in obtaining more detailed insights into the localisation and function of these proteins within fruit.
ISSN:1746-4811
1746-4811
DOI:10.1186/s13007-023-01100-3