The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis

Murray Valley Encephalitis virus (MVEV) is a mosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15⁻30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed...

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Bibliographic Details
Published in:Viruses 2018-06, Vol.10 (6), p.319
Main Authors: Russell, Jessica S, Caly, Leon, Kostecki, Renata, McGuinness, Sarah L, Carter, Glen, Bulach, Dieter, Seemann, Torsten, Stinear, Tim P, Baird, Rob, Catton, Mike, Druce, Julian
Format: Article
Language:English
Subjects:
Acids
Animals
Case reports
Cercopithecus aethiops
Cerebrospinal fluid
Cerebrospinal Fluid - virology
Child
Encephalitis
Encephalitis Virus, Murray Valley - classification
Encephalitis Virus, Murray Valley - genetics
Encephalitis Virus, Murray Valley - isolation & purification
Encephalitis, Arbovirus - virology
Epidemiology
Flavivirus
Genomes
Genotype
Genotype & phenotype
Genotypes
Health care
Health surveillance
Hospitals
Humans
Immunology
Infections
Infectious diseases
Intensive care
isolation
Laboratories
Murray Valley encephalitis
NMR
Northern Territory
Nuclear magnetic resonance
Phylogeny
Polymerase Chain Reaction
Public health
Sequence Homology
Vero Cells
Virus Cultivation
Viruses
Whole Genome Sequencing
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Summary:Murray Valley Encephalitis virus (MVEV) is a mosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15⁻30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed using both MVEV-specific real-time, and Pan- conventional, Polymerase Chain Reaction (PCR), with confirmation by Sanger sequencing. Subsequent isolation, the first from CSF, was conducted in Vero cells and the observed cytopathic effect was confirmed by increasing viral titre in the real-time PCR. Isolation allowed for full genome sequencing using the Scriptseq V2 RNASeq library preparation kit. A consensus genome for VIDRL-MVE was generated and phylogenetic analysis identified it as Genotype 2. This is the first reported isolation, and full genome sequencing of MVEV from CSF. It is also the first time Genotype 2 has been identified in humans. As such, this case has significant implications for public health surveillance, epidemiology, and the understanding of MVEV evolution.
ISSN:1999-4915
1999-4915
DOI:10.3390/v10060319