Effect of Low Power Laser Irradiation on the Ability of Cell Growth and Myogenic Differentiation of Myoblasts Cultured In Vitro

As a therapeutic modality, low power laser irradiation (LPLI) has been used clinically in the treatment of skeletal muscle injuries and other myopathic conditions, but the cellular and molecular mechanisms attributed to this therapy were still unclear. Myoblasts are a type of myogenic stem cells qui...

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Published in:International journal of photoenergy 2014-01, Vol.2014 (2014), p.1-8
Main Authors: Liang, Jie, Shen, Ben-Jian, Fu, Xiaobing, Wang, Chang-Zhen, Chen, Peng, Wang, Xiao-Ying, Li, Shao-Dan, Zhang, Cui-Ping, Kang, Hong-Xiang
Format: Article
Language:English
Subjects:
Cell cycle
Cell growth
Cellular
Differentiation
Gene expression
In vitro testing
Irradiation
Lasers
Medical research
Muscles
Rodents
Surgical implants
Wound healing
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container_issue 2014
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container_title International journal of photoenergy
container_volume 2014
creator Liang, Jie
Shen, Ben-Jian
Fu, Xiaobing
Wang, Chang-Zhen
Chen, Peng
Wang, Xiao-Ying
Li, Shao-Dan
Zhang, Cui-Ping
Kang, Hong-Xiang
description As a therapeutic modality, low power laser irradiation (LPLI) has been used clinically in the treatment of skeletal muscle injuries and other myopathic conditions, but the cellular and molecular mechanisms attributed to this therapy were still unclear. Myoblasts are a type of myogenic stem cells quiescence in mature skeletal muscle fibers and are considered as the source cells during the regenerating process. The purpose of this paper was to investigate the effects of LPLI on the proliferation and myogenic differentiation of the cultured myoblasts and to find out the major candidates responsible for LPLI-induced muscle regeneration in vivo. In this study, primary rat myoblasts were exposed to helium-neon (He-Ne) laser. Cell proliferation, differentiation, and the cellular responses to LPLI were monitored by using morphological observation and molecular biological methods. It was found that LPLI at a certain fluence could increase the cell growth potential for myoblasts and further induce more cells entering into S phase of the mitotic cycle as indicated by high levels of bromodeoxyuridine (BrdU) incorporation, while at the same time inhibiting their in vitro differentiation and decreasing the expression of myogenic regulatory genes to a certain extent. Taken together, these results provide experimental evidence for the clinical applications of LPLI in regenerating skeletal muscle.
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subjects Cell cycle
Cell growth
Cellular
Differentiation
Gene expression
In vitro testing
Irradiation
Lasers
Medical research
Muscles
Rodents
Surgical implants
Wound healing
title Effect of Low Power Laser Irradiation on the Ability of Cell Growth and Myogenic Differentiation of Myoblasts Cultured In Vitro
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