Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

• Published in: Scientific reports 2023-04, Vol.13 (1), p.6749-15, Article 6749
• Main Authors: Ruraż, Karolina, Przemieniecki, Sebastian Wojciech, Piwowarczyk, Renata
• Format: Article
• Language: English
• Subjects: 631/158; 631/326; 631/449; Ascomycota - genetics; Bacteria; Developmental stages; Flowers; Flowers & plants; Flowers - microbiology; Humanities and Social Sciences; Microbial activity; Microbiomes; Microorganisms; multidisciplinary; Mycobiome; Orobanchaceae - genetics; Orobanche; Orobanche - genetics; Parasitic plants; Plant reproductive structures; Plants - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Science; Science (multidisciplinary); Stigmas (botany)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-33676-0

Little is known about the microbiomes of flower parts, and even less information is available regarding these microorganisms’ colonization of specific niches in parasitic plants. We investigate the temporal interspecies dynamics of the parasitic plants microbiome of flower stigmas in two stages of development: immature stigmas in flower buds and mature stigmas in opened flowers. We compared two related holoparasitic Orobanche species from localities approximately 90 km apart and characterize their bacterial and fungal communities using 16S rRNA gene and ITS sequences, respectively. We identified from 127 to over 228 OTUs per sample for fungi, sequences belonging to genera: Aureobasidium , Cladosporium , Malassezia , Mycosphaerella , and Pleosporales, constituting approximately 53% of the community in total. In the bacterial profile, we recorded 40 to over 68 OTUs per sample consisting of Enterobacteriaceae, and genera Cellulosimicrobium , Pantoea , and Pseudomonas spp., with an approximately 75% frequency. In microbial communities, higher numbers of OTUs colonizing mature stigmas were recorded than in immature. This implies that the dynamics and concurrence of microbial communities were different between O. alsatica and O. bartlingii and underwent significant changes during flower development. To the best of our knowledge, is the first study of the interspecies and temporal dynamics of the bacterial and fungal microbiomes of pistil stigmas in flowers.