DNA-SIP delineates unique microbial communities in the rhizosphere of the hyperaccumulator Sedum alfredii which are beneficial to Cd phytoextraction

• Published in: Ecotoxicology and environmental safety 2024-03, Vol.272, p.116016-116016, Article 116016
• Main Authors: Hu, Lanfang, Tan, Xingyan, Lu, Lingli, Meng, Xiangtian, Li, Yaying, Yao, Huaiying
• Format: Article
• Language: English
• Subjects: Bacteria; DNA-SIP; Hyperaccumulators; Metal; Phytoremediation; Rhizosphere
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116016

Rhizo-microbe recruited by hyperaccumulating plants are crucial for the extraction of metals from contaminated soils. It is important, but difficult, to identify the specific rhizosphere microbes of hyperaccumulators shaped by root exudation. Continuous 13CO2 labeling, microbial DNA-based stable isotope probing (DNA-SIP), and high throughput sequencing were applied to identify those rhizosphere microorganisms using exudates from the Cd hyperaccumulator Sedum alfredii. In contrast to its non-hyperaccumulating ecotype (NAE), the hyperaccumulating ecotype (HAE) of S. alfredii strongly changed the rhizosphere environment and extracted a 5-fold higher concentration of Cd from contaminated soil. Although both HAE and NAE harbored Streptomyces, Massilia, Bacillus, and WPS-2 Uncultured Bacteria with relative abundance of more than 1% in the rhizosphere associated with plant growth and immunity, the HAE rhizosphere specifically recruited Rhodanobacter (2.66%), Nocardioides (1.16%), and Burkholderia (1.01%) through exudates to benefit the extraction of Cd from soil. Different from the bacterial network with weak cooperation in the NAE rhizosphere, a closed-loop bacterial network shaped by exudates was established in the HAE rhizosphere to synergistically resist Cd. This research reveals a specific rhizosphere bacterial community induced by exudates assisted in the extraction of Cd by S. alfredii and provides a new perspective for plant regulation of the rhizo-microbe community beneficial for optimizing phytoremediation. [Display omitted] Hyperaccumulator S. alfredii (HAE) absorbed more Cd than non-hyperaccumulator (NAE).The HAE and NAE rhizosphere harbored common bacterial genera in Cd-contaminated soil.The HAE rhizosphere recruited specific bacterial genera with a closed-loop network.