Evaluation of the Reparative Effect of Sinomenine in an Acetaminophen-Induced Liver Injury Model

• Published in: Current issues in molecular biology 2024-01, Vol.46 (1), p.923-933
• Main Authors: Kayalı, Ahmet, Bora, Ejder Saylav, Acar, Hüseyin, Erbaş, Oytun
• Format: Article
• Language: English
• Subjects: Acetaminophen; BMP-7; drug-induced hepatotoxicity; Sinomenine
• ISSN: 1467-3045; 1467-3037; 1467-3045
• DOI: 10.3390/cimb46010059

Due to its rising global prevalence, liver failure treatments are urgently needed. Sinomenine (SIN), an alkaloid from sinomenium acutum, is being studied for its liver-repair properties due to Acetaminophen (APAP) overdose. SIN's effect on APAP-induced hepatotoxicity in rats was examined histologically and biochemically. Three groups of 30 adult male Wistar rats were created: control, APAP-only, and APAP + SIN. Histopathological and biochemical analyses were performed on liver samples after euthanasia. SIN is significantly protected against APAP damage. Compared to APAP-only, SIN reduced cellular injury and preserved hepatocellular architecture. The APAP + SIN Group had significantly lower ALT, MDA, and GSH levels, protecting against hepatocellular damage and oxidative stress. SIN also had dose-dependent antioxidant properties. When examining critical regulatory proteins, SIN partially restored Sirtuin 1 (SIRT1) levels. While BMP-7 levels were unaffected, histopathological evidence and hepatocyte damage percentages supported SIN's liver-restorative effect. SIN protected and repaired rats' livers from APAP-induced liver injury. This study suggests that SIN may treat acute liver damage, warranting further research into its long-term effects, optimal dosage, and clinical applications. These findings aid liver-related emergency department interventions and life-saving treatments.