Osteogenic Activities of Trifolirhizin as a Bioactive Compound for the Differentiation of Osteogenic Cells

Plant extracts are widely used as traditional medicines. Aiton-derived natural compounds exert various beneficial effects, such as anti-inflammatory, anticancer, antioxidant, and antiregenerative activities, through their bioactive compounds, including flavonoids and alkaloids. In the present study,...

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Published in:International journal of molecular sciences 2023-12, Vol.24 (23), p.17103
Main Authors: Yun, Hyung-Mun, Cho, Mi Hyeon, Jeong, Hoibin, Kim, Soo Hyun, Jeong, Yun Hee, Park, Kyung-Ran
Format: Article
Language:English
Subjects:
Biological products
bone
Bone diseases
Cancer
Collagen
Cytotoxicity
differentiation
Extracellular matrix
Flavonoids
Isoflavones
Kinases
Mineralization
Morphology
Muscle proteins
NMR
Nuclear magnetic resonance
osteoblast
Osteoporosis
Phosphatases
Phosphorylation
Polymerization
Protein kinases
Proteins
RUNX2
Sophora flavescens
Transcription factors
trifolirhizin
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Summary:Plant extracts are widely used as traditional medicines. Aiton-derived natural compounds exert various beneficial effects, such as anti-inflammatory, anticancer, antioxidant, and antiregenerative activities, through their bioactive compounds, including flavonoids and alkaloids. In the present study, we investigated the biological effects of an -derived flavonoid, trifolirhizin (trifol), on the stimulation of osteogenic processes during osteoblast differentiation. Trifol (>98% purity) was successfully isolated from the root of and characterized. Trifol did not exhibit cellular toxicity in osteogenic cells, but promoted alkaline phosphatase (ALP) staining and activity, with enhanced expression of the osteoblast differentiation markers, including , , and . Trifol induced nuclear runt-related transcription factor 2 (RUNX2) expression during the differentiation of osteogenic cells, and concomitantly stimulated the major osteogenic signaling proteins, including GSK3β, β-catenin, and Smad1/5/8. Among the mitogen-activated protein kinases (MAPKs), Trifol activated JNK, but not ERK1/2 and p38. Trifol also increased the osteoblast-mediated bone-forming phenotypes, including transmigration, F-actin polymerization, and mineral apposition, during osteoblast differentiation. Overall, trifol exhibits bioactive activities related to osteogenic processes via differentiation, migration, and mineralization. Collectively, these results suggest that trifol may serve as an effective phytomedicine for bone diseases such as osteoporosis.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242317103