Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles

The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression profiles i...

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Bibliographic Details
Published in:Scientific reports 2018-09, Vol.8 (1), p.13919-13, Article 13919
Main Authors: Johnson, Eric K., Matkovich, Scot J., Nerbonne, Jeanne M.
Format: Article
Language:English
Subjects:
38/39
38/90
38/91
631/208/191/2018
631/208/199
631/208/514/1949
Adult
Cardiovascular disease
Cardiovascular diseases
Differential Expression Analysis
Epigenetics
Female
Gender differences
Gene expression
Gene Expression Profiling
Genomes
Heart
Heart Atria - metabolism
Heart Ventricles - metabolism
Humanities and Social Sciences
Humans
lncRNA Expression Profiles
Male
Medicine
Middle Aged
multidisciplinary
Myocardial Infarction Associated Transcript (MIAT)
Ontology
Paired Tissue Samples
Physiology
Regional differences
RNA, Long Noncoding - genetics
RNA, Messenger - genetics
Science
Science (multidisciplinary)
Septum
Sequence Analysis, RNA
Sex differences
Signal Transduction
Transplants & implants
Unsupervised Hierarchical Clustering
Ventricle
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Summary:The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression profiles in the left (LA) and right (RA) atria, the left (LV) and right (RV) ventricles, and the interventricular septum (IVS) of non-failing human hearts (N = 8). Analysis of paired atrial and ventricular samples (n = 40) identified 5,747 mRNAs and 2,794 lncRNAs that were differentially (>1.5 fold; FDR 2,300 mRNAs and >1,200 lncRNAs, corresponding to 17–28% of the total transcripts, were differentially expressed. Heterogeneities in mRNA/lncRNA expression profiles in the LA and RA, as well as in the LV, RV and IVS, were also revealed, although the numbers of differentially expressed transcripts were substantially smaller. Gender differences in mRNA and lncRNA expression profiles were also evident in non-failing human atria and ventricles. Gene ontology classification of differentially expressed gene sets revealed chamber-specific enrichment of numerous signaling pathways.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-32154-2