Relationship between minimal residual disease measured by multiparametric flow cytometry prior to allogeneic hematopoietic stem cell transplantation and outcome in children with acute lymphoblastic leukemia

The presence of minimal residual disease detected by polymerase chain reaction techniques prior to allogeneic hematopoietic stem cell transplantation has proven to be an independent prognostic factor for poor outcome in children with acute lymphoblastic leukemia. The aim of this study was to ascerta...

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Bibliographic Details
Published in:Haematologica (Roma) 2010-06, Vol.95 (6), p.936-941
Main Authors: ELORZA, Izaskun, PALACIO, Carlos, DAPENA, Jose Luis, GALLUR, Laura, SANCHEZ DE TOLEDO, José, DIAZ DE HEREDIA, Cristina
Format: Article
Language:English
Subjects:
Adolescent
Biological and medical sciences
Child
Child, Preschool
Female
Flow Cytometry - methods
Follow-Up Studies
Hematologic and hematopoietic diseases
Hematopoietic Stem Cell Transplantation - methods
Humans
Infant
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
Neoplasm, Residual
Original
Precursor Cell Lymphoblastic Leukemia-Lymphoma - mortality
Precursor Cell Lymphoblastic Leukemia-Lymphoma - radiotherapy
Precursor Cell Lymphoblastic Leukemia-Lymphoma - surgery
Risk Factors
Survival Rate - trends
Transplantation, Homologous
Treatment Outcome
Whole-Body Irradiation - trends
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Summary:The presence of minimal residual disease detected by polymerase chain reaction techniques prior to allogeneic hematopoietic stem cell transplantation has proven to be an independent prognostic factor for poor outcome in children with acute lymphoblastic leukemia. The aim of this study was to ascertain whether the presence of minimal residual disease detected by multiparametric flow cytometry prior to allogeneic hematopoietic stem cell transplantation is related to outcome in children acute lymphoblastic leukemia. Minimal residual disease was quantified by multiparametric flow cytometry at a median of 10 days prior to hematopoietic stem cell transplantation in 31 children (age range, 10 months to 16 years) with acute lymphoblastic leukemia. Thirteen patients were transplanted in first remission. Stem cell donors were HLA-identical siblings in 8 cases and matched unrelated donors in 23. Twenty-six children received a total body irradiation-containing conditioning regimen. According to the level of minimal residual disease, patients were divided into two groups: minimal residual disease-positive (>or=0.01%) (n=10) and minimal residual disease-negative (
ISSN:0390-6078
1592-8721
DOI:10.3324/haematol.2009.010843