QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms

The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the ability to catalyze the hydrolysis of the...

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Published in:Lipids in health and disease 2012-09, Vol.11 (1), p.124-124, Article 124
Main Authors: Wang, Haikuan, Wang, Xiaojie, Li, Xiaolu, Zhang, Yehong, Dai, Yujie, Guo, Changlu, Zheng, Heng
Format: Article
Language:English
Subjects:
Bacteria - enzymology
Catalysis
China
Colleges & universities
Detergents, Synthetic
Enzymes
Ester
Esters - chemistry
Esters - metabolism
GFA
Hydrolysis
Kinetics
Lipase
Lipase - chemistry
Lipase - metabolism
Lipase activity
Microbiology
Microorganisms
Oils & fats
Plant Oils - chemistry
QSAR
Quantitative Structure-Activity Relationship
Soap and cleaning agents industry
Substrate Specificity
Triglycerides
Triglycerides - chemistry
Triglycerides - metabolism
Vegetable oils
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Summary:The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the ability to catalyze the hydrolysis of the glycerides, methyl esters, ethyl esters, especially for triglycerides, which shows that they have broad substrate spectra, and this property is very important for them to be used in detergent industry. Three QSAR models were built for L-A1, L-A2 and L-A3 respectively with GFA using Discovery studio 2.1. The models equations 1, 2 and 3 can explain 95.80%, 97.45% and 97.09% of the variances (R(2)(adj)) respectively while they could predict 95.44%, 89.61% and 93.41% of the variances (R(2)(cv)) respectively. With these models the hydrolysis activities of these lipases to mixed esters were predicted and the result showed that the predicted values are in good agreement with the measured values, which indicates that this method can be used as a simple tool to predict the lipase activities for single or mixed esters.
ISSN:1476-511X
1476-511X
DOI:10.1186/1476-511X-11-124