Identification of LINC02310 as an enhancer in lung adenocarcinoma and investigation of its regulatory network via comprehensive analyses

• Published in: BMC medical genomics 2020-12, Vol.13 (1), p.185-185, Article 185
• Main Authors: Zhao, Wenyuan, Wang, Jun, Luo, Qingxi, Peng, Wei, Li, Bin, Wang, Lei, Zhang, Chunfang, Duan, Chaojun
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Adenocarcinoma of Lung - genetics; Adenocarcinoma of Lung - mortality; Biomarkers; Biomarkers, Tumor; Cancer; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - mortality; ceRNA regulatory network; Chemotherapy; Cholecystokinin; Colonies; Datasets; Datasets as Topic; Development and progression; Experiments; Gene expression; Gene Expression Regulation, Neoplastic; Gene Ontology; Gene Regulatory Networks; Genetic aspects; Genetic markers; Genomes; Health aspects; Humans; Identification and classification; Kaplan-Meier Estimate; LADC; LncRNA; Lung cancer; Lung cancer, Non-small cell; Lung Neoplasms - genetics; Lung Neoplasms - mortality; Neoplasm Staging; Non-coding RNA; Non-small cell lung carcinoma; Oncology, Experimental; PPI; Prognosis; Protein Interaction Maps; Radiation therapy; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; RNA, Neoplasm - genetics; Sincalide - analysis; Small cell lung carcinoma; Survival analysis; TCGA; Tumor Stem Cell Assay; Tumors; Values
• ISSN: 1755-8794; 1755-8794
• DOI: 10.1186/s12920-020-00834-6

Lung adenocarcinoma (LADC) is a major subtype of non-small cell lung cancer and has one of the highest mortality rates. An increasing number of long non-coding RNAs (LncRNAs) were reported to be associated with the occurrence and progression of LADC. Thus, it is necessary and reasonable to find new prognostic biomarkers for LADC among LncRNAs. Differential expression analysis, survival analysis, PCR experiments and clinical feature analysis were performed to screen out the LncRNA which was significantly related to LADC. Its role in LADC was verified by CCK-8 assay and colony. Furthermore, competing endogenous RNA (ceRNA) regulatory network construction, enrichment analysis and protein-protein interaction (PPI) network construction were performed to investigate the downstream regulatory network of the selected LncRNA. A total of 2431 differentially expressed LncRNAs (DELncRNAs) and 2227 differentially expressed mRNAs (DEmRNAs) were from The Cancer Genome Atlas database. Survival analysis results indicated that lnc-YARS2-5, lnc-NPR3-2 and LINC02310 were significantly related to overall survival. Their overexpression indicated poor prognostic. PCR experiments and clinical feature analysis suggested that LINC02310 was significantly correlated with TNM-stage and T-stage. CCK-8 assay and colony formation assay demonstrated that LINC02310 acted as an enhancer in LADC. In addition, 3 targeted miRNAs of LINC02310 and 414 downstream DEmRNAs were predicted. The downstream DEmRNAs were then enriched in 405 Gene Ontology terms and 11 Kyoto Encyclopedia of Genes and Genomes pathways, which revealed their potential functions and mechanisms. The PPI network showed the interactions among the downstream DEmRNAs. This study verified LINC02310 as an enhancer in LADC and performed comprehensive analyses on its downstream regulatory network, which might benefit LADC prognoses and therapies.