PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography

Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and for entrapment of Parkia pendula seed gum (Ppe...

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Published in:Acta scientiarum. Biological sciences 2016-12, Vol.38 (3), p.291
Main Authors: Rêgo, Moacyr Jesus Barreto de Melo, Lima, Luiza Rayanna Amorim de, Longo, Andrei Figueiredo Passo, Nascimento-Filho, Gilson Alves, Carvalho Júnior, Luiz Bezera de, Beltrão, Eduardo Isidoro Carneiro
Format: Article
Language:English
Subjects:
affinity column
Biotechnology
Con A
Extraction processes
gum
interpenetrated network
Parkia pendula
Polyvinyl alcohol
purification
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Summary:Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and for entrapment of Parkia pendula seed gum (PpeG). Con A immobilization yielded approximately 30% and 0.6 M glucose solution was the minimum concentration able to elute fetuin from column. PVA-GA-PpeG column was efficiently recognized by pure P. pendula lectin (PpeL). These findings indicate that PVA-GA interpenetrated network showed to be an efficient support for lectin covalent immobilization and as affinity chromatography matrix after trapping of PpeG.
ISSN:1679-9283
1807-863X
DOI:10.4025/actascibiolsci.v38i3.31369