Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli

Shikimate is an important intermediate in the aromatic amino acid pathway, which can be used as a promising building block for the synthesis of biological compounds, such as neuraminidase inhibitor Oseltamivir (Tamiflu ). Compared with traditional methods, microbial production of shikimate has the a...

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Bibliographic Details
Published in:Microbial cell factories 2017-09, Vol.16 (1), p.167-167, Article 167
Main Authors: Gu, Pengfei, Fan, Xiangyu, Liang, Quanfeng, Qi, Qingsheng, Li, Qiang
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Blocking
Construction
Corynebacterium glutamicum
Dehydrogenases
E coli
Enzymes
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Exo-a-sialidase
Feedback
Genes
Genetic engineering
Glycerol
Health aspects
Influenza
Kinases
Low cost
Metabolic Engineering
Metabolism
Metabolites
Microorganisms
Novel technologies
Oseltamivir
Plasmids
Production methods
Review
Selectivity
Shikimate
Shikimic Acid - metabolism
Synthetic Biology
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Summary:Shikimate is an important intermediate in the aromatic amino acid pathway, which can be used as a promising building block for the synthesis of biological compounds, such as neuraminidase inhibitor Oseltamivir (Tamiflu ). Compared with traditional methods, microbial production of shikimate has the advantages of environmental friendliness, low cost, feed stock renewability, and product selectivity and diversity, thus receiving more and more attentions. The development of metabolic engineering allows for high-efficiency production of shikimate of Escherichia coli by improving the intracellular level of precursors, blocking downstream pathway, releasing negative regulation factors, and overexpressing rate-limiting enzymes. In addition, novel technologies derived from systems and synthetic biology have opened a new avenue towards construction of shikimate-producing strains. This review summarized successful and applicable strategies derived from traditional metabolic engineering and novel technologies for increasing accumulation of shikimate in E. coli.
ISSN:1475-2859
1475-2859
DOI:10.1186/s12934-017-0773-y