Homalomena pineodora essential oil nanoparticle inhibits diabetic wound pathogens

• Published in: Scientific reports 2020-02, Vol.10 (1), p.3307-11, Article 3307
• Main Authors: Rozman, Nur Amiera Syuhada, Tong, Woei Yenn, Leong, Chean Ring, Anuar, Mohd Razealy, Karim, Sabrina, Ong, Siew Kooi, Yusof, Fahmi Asyadi Md, Tan, Wen-Nee, Sulaiman, Baharuddin, Ooi, Mei Lee, Lee, Kok Chang
• Format: Article
• Language: English
• Subjects: 13; 14/28; 14/63; 631/61/54/152; 692/308/153; Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial activity; Antimicrobial agents; Araceae - chemistry; Bacteria - drug effects; Bacteria - growth & development; Chitosan; Chitosan - chemistry; Collagen; Collagen - metabolism; Diabetes; Diabetes mellitus; Diabetes Mellitus - drug therapy; Diabetes Mellitus - microbiology; Diabetes Mellitus - pathology; Drug Liberation; Essential oils; Gelation; Humanities and Social Sciences; Microbial Sensitivity Tests; multidisciplinary; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Oils & fats; Oils, Volatile - pharmacology; Oils, Volatile - therapeutic use; Particle Size; Pathogens; Science; Science (multidisciplinary); Spectroscopy, Fourier Transform Infrared; Surface charge; Ulcers; Wound Healing; Wounds; X-Ray Diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-60364-0

Essential oil of Homalomena pineodora inhibits diabetic pathogens; however, the activity was not sustainable when applied as wound dressing. This study aims to synthesise the essential oil nanoparticle using chitosan. The nanoparticles were synthesised with ion gelation method, confirmed by spectroscopic analysis. The spherical nanoparticles display a size of 70 nm, with strong surface charge of +24.10 mV. The nanoparticles showed an initial burst release followed by a slow release pattern for 72 h, following the first order of kinetic. The release behaviour was ideal for wound dressing. The antimicrobial activity was broad spectrum. The formation of nanoparticle enhanced the antimicrobial efficacy of the essential oil. The nanoparticle also showed a concentration-dependent killing behaviour on time–kill assay. In the 3D collagen wound models, the nanoparticles reduced the microbial growth by 60–80%. In conclusion, H . pineodora nanoparticles showed pharmaceutical potential in inhibiting microbial growth on diabetic ulcers.