P53 codon 11, 72, and 248 gene polymorphisms in endometriosis

Mutated p53 gene is related to the instability of cell growth and cell cycle progression. We aimed to evaluate the association between endometriosis and p53 codon 11, 72 and 248 gene polymorphisms. Women were divided into two groups: (1) moderate/severe endometriosis (n=148), and (2) non-endometrios...

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Bibliographic Details
Published in:International journal of biological sciences 2006-01, Vol.2 (4), p.188-193
Main Authors: Hsieh, Yao-Yuan, Lin, Chich-Sheng
Format: Article
Language:English
Subjects:
Arginine - genetics
Codon - genetics
DNA - blood
DNA - genetics
DNA - isolation & purification
DNA Primers
Endometriosis - genetics
Endometriosis - pathology
Female
Humans
Lysine - genetics
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Premenopause
Proline - genetics
Reference Values
Research Paper
Severity of Illness Index
Taiwan
Tumor Suppressor Protein p53 - genetics
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Summary:Mutated p53 gene is related to the instability of cell growth and cell cycle progression. We aimed to evaluate the association between endometriosis and p53 codon 11, 72 and 248 gene polymorphisms. Women were divided into two groups: (1) moderate/severe endometriosis (n=148), and (2) non-endometriosis groups (n=150). P53 gene polymorphisms include codon11 Glu/Gln or Lys (GAG->CAG or AAG), codon 72 Arg/Pro (CGC->CCC), and codon 248 Arg/Thr (CGG->TCG). These gene polymorphisms were amplified by polymerase chain reaction and detected by electrophoresis after restriction enzyme (Taq I, BstU I, Hap II) digestions. Associations between the endometriosis and p53 polymorphisms were evaluated. The distributions of p53 codon 72 polymorphisms in both groups were significantly different. The proportions of Arg homozygotes/heterozygotes/Pro homozygotes in both groups were 9.5/66.2/24.3% and 30.7/50/19.3%. The proportions of Arg/Pro alleles were 42.6/57.4% and 56/44%. The distributions of p53 codon 11 and 248 polymorphisms in both groups were non-significantly different. All individuals appeared the wild genotypes (Glu11 and Arg248 homozygotes). Association between endometriosis and p53 codon 72 polymorphism exists. P53 codon 72*Pro-related genotype and allele are related with higher susceptibility of endometriosis. P53 codon 11 and 248 polymorphisms are not related with endometriosis susceptibility.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.2.188