Functional bioactive compounds and biological activities of Spirulina platensis lipids

• Published in: Czech Journal of Food Sciences 2008-01, Vol.26 (3), p.211-222
• Main Authors: Ramadan, M.F.,Zagazig Univ. (Egypt). Biochemistry Dept, Asker, M.M.S.,National Research Center, Cairo (Egypt). Microbial Biotechnology Dept, Ibrahim, Z.K.,Cairo Univ. (Egypt). Botany Dept
• Format: Article
• Language: English
• Subjects: ACIDE GRAS; ACIDE LINOLEIQUE; ACIDE LINOLENIQUE; ACIDE PALMITIQUE; ACIDO LINILENICO; ACIDO LINOLEICO; ACIDO PALMITICO; ACIDOS GRASOS; Ammonia; antimicrobial activity; ANTIMICROBIAL PROPERTIES; ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; antiradical action; ASPERGILLUS FLAVUS; ASPERGILLUS NIGER; BACILLUS SUBTILIS; Bacteria; Bioactive compounds; blue-green microalga; CANDIDA ALBICANS; Chloroform; CHROMATOGRAPHIE EN COLONNE; CHROMATOGRAPHIE EN COUCHE MINCE; CHROMATOGRAPHIE GAZ LIQUIDE; COLUMN CHROMATOGRAPHY; CRECIMIENTO; CROISSANCE; CROMATOGRAFIA DE CAPA FINA; CROMATOGRAFIA DE COLUMNA; CROMATOGRAFIA GAS-LIQUIDO; Cyanobacteria; ESCHERICHIA COLI; FATTY ACIDS; FOSFOLIPIDOS; Free radicals; GAS LIQUID CHROMATOGRAPHY; GLICOLIPIDOS; GLYCOLIPIDE; GLYCOLIPIDS; Gram-negative bacteria; GROWTH; HPLC; http://www.fao.org/aos/agrovoc#c_14858; http://www.fao.org/aos/agrovoc#c_15940; http://www.fao.org/aos/agrovoc#c_15993; http://www.fao.org/aos/agrovoc#c_16429; http://www.fao.org/aos/agrovoc#c_24395; http://www.fao.org/aos/agrovoc#c_24469; http://www.fao.org/aos/agrovoc#c_26370; http://www.fao.org/aos/agrovoc#c_26373; http://www.fao.org/aos/agrovoc#c_26646; http://www.fao.org/aos/agrovoc#c_26756; http://www.fao.org/aos/agrovoc#c_27476; http://www.fao.org/aos/agrovoc#c_2818; http://www.fao.org/aos/agrovoc#c_28292; http://www.fao.org/aos/agrovoc#c_28427; http://www.fao.org/aos/agrovoc#c_32670; http://www.fao.org/aos/agrovoc#c_33700; http://www.fao.org/aos/agrovoc#c_3394; http://www.fao.org/aos/agrovoc#c_35586; http://www.fao.org/aos/agrovoc#c_3791; http://www.fao.org/aos/agrovoc#c_4352; http://www.fao.org/aos/agrovoc#c_4362; http://www.fao.org/aos/agrovoc#c_511; http://www.fao.org/aos/agrovoc#c_5517; http://www.fao.org/aos/agrovoc#c_5803; http://www.fao.org/aos/agrovoc#c_6724; http://www.fao.org/aos/agrovoc#c_6973; http://www.fao.org/aos/agrovoc#c_7798; IDENTIFICACION; IDENTIFICATION; INHIBICION; INHIBITION; LINOLEIC ACID; LINOLENIC ACID; LIPIDE; LIPIDOS; LIPIDS; Microorganisms; PALMITIC ACID; PHOSPHATIDE; PHOSPHOLIPIDS; PROPIEDADES ANTIMICROBIANAS; PROPRIETE ANTIMICROBIENNE; PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE; Scavenging; SEPARACION; SEPARATING; SEPARATION; SPIRULINA PLATENSIS; STAPHYLOCOCCUS AUREAS; STAPHYLOCOCCUS AUREUS; THIN LAYER CHROMATOGRAPHY; TOCOFEROLES; TOCOPHEROL; TOCOPHEROLS
• ISSN: 1212-1800; 1805-9317
• DOI: 10.17221/2567-cjfs

The compositions and concentrations of lipid classes, fatty acids and tocopherols were determined in the lipids from Spirulina platensis. Total lipids (TL) recovered using chloroform : methanol (2:1, v/v) were found to be 163.5 g/kg (on dry weight basis). The level of neutral lipids was the highest, followed by glycolipids and phospholipids, respectively. Among TL and lipid classes, palmitic, gamma-linolenic and linoleic acids were the dominating fatty acids. Compared with the neutral lipids, the polar fractions were generally characterised by higher percentages of saturated fatty acids. The recovered lipids were characterised by high percentage of tocopherols, wherein alpha-tocopherol constituted about 73% of total tocopherols present, the rest having been gamma-tocopherol. Spirulina platensis lipids exhibited a strong radical scavenging activity towards stable DPPH free radicals, whereas 27% of DPPH radicals were quenched after 2 h incubation. TL and lipid classes inhibited the growth of different microorganisms except gram-negative bacteria. At high concentrations, the tested lipids appeared more effective against A. niger (28.3+/-1.53 mm).