Chemical Composition of Natural Hydrolates and Their Antimicrobial Activity on Arcobacter -Like Cells in Comparison with Other Microorganisms

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-11, Vol.25 (23), p.5654
• Main Authors: Šilha, David, Švarcová, Karolína, Bajer, Tomáš, Královec, Karel, Tesařová, Eliška, Moučková, Kristýna, Pejchalová, Marcela, Bajerová, Petra
• Format: Article
• Language: English
• Subjects: A549 Cells; Acetic acid; Anti-Infective Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Arcobacter - drug effects; Bacteria; Biofilms; Candida albicans - drug effects; Cell lines; Cell Proliferation; Chemical analysis; Chemical composition; Chromatography; Cytotoxicity; Distillation; Eugenol; Flame ionization detectors; Foeniculum; Foeniculum vulgare; Gas chromatography; Gram-positive bacteria; Humans; hydrolates; Ionization; Laurus nobilis; Lavandula - chemistry; Lavandula angustifolia; Linalool; Lung Neoplasms - drug therapy; Mass spectrometry; Mass spectroscopy; Microorganisms; Oils & fats; Oils, Volatile - pharmacology; Plant Oils - pharmacology; Scientific imaging; Steam distillation; Syzygium aromaticum; Terpineol
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25235654

Hydrolates obtained via the hydrodistillation and steam distillation of Mill., L., Mill., and L. were analyzed by gas chromatography with flame ionization detector (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). Additionally, the hydrolates were evaluated for antimicrobial activity (disk-diffusion and microdilution method), influence on biofilm formation (Christensen method) and cytotoxicity of concentrated hydrolates against human cell lines (A549) by xCELLigence system. Using chemical analysis, 48, 9, 13 and 33 different components were detected in lavender, clove, fennel and laurel hydrolates, respectively. Lavender hydrolate contained the largest proportion of 1,8-cineol, linalool furanoxide, and linalool. The main components of laurel hydrolate were 1,8-cineol, 4-terpineol and α-terpineol. Fenchone and estragole were the most abundant in fennel hydrolate, and eugenol and eugenyl acetate in clove hydrolate. Concentrated hydrolates showed significant antimicrobial activity. Clove hydrolate was among the most antimicrobially active agents, most preferably against . , with an inhibition zone up to 23.5 mm. Moreover, concentrated hydrolates did not show any cytotoxic effect again8 st human A549 cells. In the presence of the non-concentrated hydrolates, significantly reduced biofilm formation was observed; however, with concentrated clove hydrolate, there was an increase in biofilm formation, e.g., of . , . , and . . Research shows new findings about hydrolates that may be important in natural medicine or for preservation purposes.