Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study

• Published in: Cell journal (Yakhteh) 2024-09, Vol.26 (7), p.454-464
• Main Authors: Shahraki, Hojat, Gheydari, Mohammad Esmail, Mohammadi, Mohammad Hossein, Bashash, Davood, Ghorbani, Mohammad, Mirsattari, Dariush, Olazadeh, Keyvan, Amiri, Vahid, Sargazi-Aval, Omolbanin, Hamidpour, Mohsen
• Format: Article
• Language: English
• Subjects: acute coronary syndrome; integrins; macrophage-1 antigen; platelet-monocyte aggregates; thromboinflammation
• ISSN: 2228-5806; 2228-5814
• DOI: 10.22074/cellj.2024.2024525.1527

Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P