Quality of horse F(ab') 2 antitoxins and anti-rabies immunoglobulins: protein content and anticomplementary activity

Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and...

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Bibliographic Details
Published in:The journal of venomous animals and toxins including tropical diseases 2018-06, Vol.24 (1), p.16-16, Article 16
Main Authors: Squaiella-Baptistão, Carla Cristina, Magnoli, Fábio Carlos, Marcelino, José Roberto, Sant'Anna, Osvaldo Augusto, Tambourgi, Denise V
Format: Article
Language:English
Subjects:
Aggregates
Anaphylatoxins
Anti-rabies
Antitoxins
Botulinum toxin
Botulism
Clinical trials
Clostridium botulinum
Complement activation
Complement component C5a
Complement system
Contaminants
Data processing
Diphtheria
Disease
F(ab’)2 fragment
Food contamination
Gel electrophoresis
Heterologous immunoglobulin
Immunization
Immunoglobulin G
Immunoglobulins
Immunotherapy
Infectious diseases
Medical treatment
Molecular chains
Patients
Prophylaxis
Protein profile
Proteins
Quality control
Rabies
Side effects
Sodium lauryl sulfate
Statistical analysis
Statistical methods
Tetanus
Variance analysis
Venom
Waterborne diseases
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Summary:Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and on the quality control are necessary to reduce the amount of protein aggregates. These may trigger adverse reactions in patients by activating the complement system and inducing the generation of anaphylatoxins. Herein, we used immunochemical methods to predict the quality of horse F(ab') anti-botulinum AB, anti-diphtheric, antitetanic and anti-rabies immunoglobulins, in terms of amount of proteins and protein aggregates. Samples were submitted to protein quantification, SDS-PAGE, Western blot analysis and molecular exclusion chromatography. The anticomplementary activity was determined in vitro by detecting the production of C5a/C5a desArg, the most potent anaphylatoxin. Data were analyzed by one-way ANOVA followed by Tukey's post-test, and differences were considered statistically significant when  
ISSN:1678-9199
1678-9199
DOI:10.1186/s40409-018-0153-z