Antibacterial Hydrogels Derived from Poly(γ-glutamic acid) Nanofibers

Biocompatible hydrogels with antibacterial properties derived from γ-polyglutamic acid (γ-PGA) were prepared from bulk and electrospun nanofibers. The antibacterial drugs loaded in these hydrogels were triclosan (TCS), chlorhexidine (CHX) and polyhexamethylene biguanide (PHMB); furthermore, bacterio...

Full description

Saved in:
Bibliographic Details
Published in:Gels 2022-02, Vol.8 (2), p.120
Main Authors: Kasbiyan, Hamidreza, Yousefzade, Omid, Simiand, Estelle, Saperas, Núria, Del Valle, Luis J, Puiggalí, Jordi
Format: Article
Language:English
Subjects:
Acids
antibacterial properties
Antibiotics
Bacterial infections
bacteriophages
Biocompatibility
Chlorhexidine
Contact angle
Crosslinking
Drug delivery systems
drug release
Electrospinning
Flow velocity
Glutamic acid
Hydrogels
Hydrophobicity
Infections
Microorganisms
Molecular weight
Morphology
Nanofibers
Phages
Polyhexanide
Polymer solubility
Solvents
Surface roughness
Viscosity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Biocompatible hydrogels with antibacterial properties derived from γ-polyglutamic acid (γ-PGA) were prepared from bulk and electrospun nanofibers. The antibacterial drugs loaded in these hydrogels were triclosan (TCS), chlorhexidine (CHX) and polyhexamethylene biguanide (PHMB); furthermore, bacteriophages were loaded as an alternative antibacterial agent. Continuous and regular γ-PGA nanofibers were successfully obtained by the electrospinning of trifluoroacetic acid solutions in a narrow polymer concentration range and restricted parameter values of flow rate, voltage and needle-collector distance. Hydrogels were successfully obtained by using cystamine as a crosslinking agent following previous published procedures. A closed pore structure was characteristic of bulk hydrogels, whereas an open but structurally consistent structure was found in the electrospun hydrogels. In this case, the morphology of the electrospun nanofibers was drastically modified after the crosslinking reaction, increasing their diameter and surface roughness according to the amount of the added crosslinker. The release of TCS, CHX, PHMB and bacteriophages was evaluated for the different samples, being results dependent on the hydrophobicity of the selected medium and the percentage of the added cystamine. A high efficiency of hydrogels to load bacteriophages and preserve their bactericide activity was demonstrated too.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels8020120