Localization and regulation of yeast aldehyde dehydrogenase Ald4p structures

Previously, we identified yeast strains, namely SWORD, showing more robust Ald4p-GFP filament formation than the typical ALD4::GFP strains. Here, we report that Ald4p-GFP in SWORD strains favorably polymerize into gigantic structures in the cytoplasm, despite the enzyme being established as a mitoch...

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Bibliographic Details
Published in:Heliyon 2024-10, Vol.10 (20), p.e39048, Article e39048
Main Authors: Nasalingkhan, Channarong, Sirinonthanawech, Naraporn, Sato, Brian K., Wilhelm, James E., Noree, Chalongrat
Format: Article
Language:English
Subjects:
Ald4p
Aldehyde dehydrogenase
Enzyme assembly
Yeast
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Summary:Previously, we identified yeast strains, namely SWORD, showing more robust Ald4p-GFP filament formation than the typical ALD4::GFP strains. Here, we report that Ald4p-GFP in SWORD strains favorably polymerize into gigantic structures in the cytoplasm, despite the enzyme being established as a mitochondrial resident. In addition, we have found that nocodazole, a microtubule destabilizer, has no effect on Ald4p high-order assembly, suggesting no direct association between microtubule dynamics and Ald4p structure formation. Ald4p assembly cannot be induced by sodium azide treatment, indicating that ATP is not a primary effector of Ald4p polymerization. Interestingly, addition of exogenous acetaldehyde, a substrate of the enzyme, can significantly enhance the structure formation of Ald4p, implying that structure formation may be related to enzymatic activity. [Display omitted]
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2024.e39048