Diet‐induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion

Introduction Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion. Methods Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a...

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Published in:Endocrinology, diabetes & metabolism diabetes & metabolism, 2020-01, Vol.3 (1), p.e00095-n/a
Main Authors: Saade, Marie‐Béatrice, Cahu, Armelle, Moriez, Raphaël, Neunlist, Michel, Blat, Sophie
Format: Article
Language:English
Subjects:
Diet
Digital cameras
endocrine pancreas
Endocrinology and metabolism
ex vivo model
Food and Nutrition
Glucose
Human health and pathology
Immunoglobulins
Insulin
Life Sciences
Motility
Nervous system
Obesity
Original
Pancreas
Western diet
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Summary:Introduction Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion. Methods Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a Western diet (WD group) for 12 weeks. Pancreas nervous system density, PINS phenotype and pancreas anatomy were analysed by immunohistochemistry at T0 and in adulthood (ND and WD groups). Insulin secretion was also studied in these 3 groups using a new model of ex vivo pancreatic culture, where PINS was stimulated by nicotinic and nitrergic agonists with and without antagonists. Insulin was assayed in supernatants by ELISA. Results Pancreas nervous system density decreased with age in ND (P 
ISSN:2398-9238
2398-9238
DOI:10.1002/edm2.95