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Diet‐induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion
Introduction Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion. Methods Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a...
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| Published in: | Endocrinology, diabetes & metabolism diabetes & metabolism, 2020-01, Vol.3 (1), p.e00095-n/a |
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| creator | Saade, Marie‐Béatrice Cahu, Armelle Moriez, Raphaël Neunlist, Michel Blat, Sophie |
| description | Introduction
Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion.
Methods
Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a Western diet (WD group) for 12 weeks. Pancreas nervous system density, PINS phenotype and pancreas anatomy were analysed by immunohistochemistry at T0 and in adulthood (ND and WD groups). Insulin secretion was also studied in these 3 groups using a new model of ex vivo pancreatic culture, where PINS was stimulated by nicotinic and nitrergic agonists with and without antagonists. Insulin was assayed in supernatants by ELISA.
Results
Pancreas nervous system density decreased with age in ND (P |
| doi_str_mv | 10.1002/edm2.95 |
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Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion.
Methods
Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a Western diet (WD group) for 12 weeks. Pancreas nervous system density, PINS phenotype and pancreas anatomy were analysed by immunohistochemistry at T0 and in adulthood (ND and WD groups). Insulin secretion was also studied in these 3 groups using a new model of ex vivo pancreatic culture, where PINS was stimulated by nicotinic and nitrergic agonists with and without antagonists. Insulin was assayed in supernatants by ELISA.
Results
Pancreas nervous system density decreased with age in ND (P < .01) but not in WD mice (P = .08). Western diet decreased the PINS nitrergic component as compared to normal diet (P < .01) but it did not modify its cholinergic component (P = .50). Nicotinic PINS stimulation induced greater insulin secretion in ND compared to WD mice (P < .001) whereas nitrergic stimulation significantly decreased insulin secretion in ND mice (P < .001) and tended to increase insulin secretion in WD mice (P = .08). Endocrine pancreas anatomy was not modified by the Western diet as compared to the normal diet (P = .93).
Conclusions
Early Western diet induced neuronal density and phenotype changes in PINS that might be involved in the pancreas insulin secretion dysfunctions associated with obesity.
Obesity starting in the young age has deep consequences on the maturation of the pancreas innervation (density and intrinsic nervous system phenotype) and the nervous control of insulin secretion. Western diet in childhood altered the normal course of pancreatic intrinsic nervous system maturation and impaired nicotinic control of insulin secretion.</description><identifier>ISSN: 2398-9238</identifier><identifier>EISSN: 2398-9238</identifier><identifier>DOI: 10.1002/edm2.95</identifier><identifier>PMID: 31922022</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Diet ; Digital cameras ; endocrine pancreas ; Endocrinology and metabolism ; ex vivo model ; Food and Nutrition ; Glucose ; Human health and pathology ; Immunoglobulins ; Insulin ; Life Sciences ; Motility ; Nervous system ; Obesity ; Original ; Pancreas ; Western diet</subject><ispartof>Endocrinology, diabetes & metabolism, 2020-01, Vol.3 (1), p.e00095-n/a</ispartof><rights>2019 The Authors. published by John Wiley & Sons Ltd</rights><rights>2019 The Authors. Endocrinology, Diabetes & Metabolism published by John Wiley & Sons Ltd.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4635-b6e9f7c037ab7c9f8b4d3358a1a992e99c605667e5a1d465ae66a446a71118c13</citedby><cites>FETCH-LOGICAL-c4635-b6e9f7c037ab7c9f8b4d3358a1a992e99c605667e5a1d465ae66a446a71118c13</cites><orcidid>0000-0002-2138-7078 ; 0000-0003-1892-5071</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2334138883/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2334138883?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,11562,25753,27924,27925,37012,37013,44590,46052,46476,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31922022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03619019$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Saade, Marie‐Béatrice</creatorcontrib><creatorcontrib>Cahu, Armelle</creatorcontrib><creatorcontrib>Moriez, Raphaël</creatorcontrib><creatorcontrib>Neunlist, Michel</creatorcontrib><creatorcontrib>Blat, Sophie</creatorcontrib><title>Diet‐induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion</title><title>Endocrinology, diabetes & metabolism</title><addtitle>Endocrinol Diabetes Metab</addtitle><description>Introduction
Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion.
Methods
Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a Western diet (WD group) for 12 weeks. Pancreas nervous system density, PINS phenotype and pancreas anatomy were analysed by immunohistochemistry at T0 and in adulthood (ND and WD groups). Insulin secretion was also studied in these 3 groups using a new model of ex vivo pancreatic culture, where PINS was stimulated by nicotinic and nitrergic agonists with and without antagonists. Insulin was assayed in supernatants by ELISA.
Results
Pancreas nervous system density decreased with age in ND (P < .01) but not in WD mice (P = .08). Western diet decreased the PINS nitrergic component as compared to normal diet (P < .01) but it did not modify its cholinergic component (P = .50). Nicotinic PINS stimulation induced greater insulin secretion in ND compared to WD mice (P < .001) whereas nitrergic stimulation significantly decreased insulin secretion in ND mice (P < .001) and tended to increase insulin secretion in WD mice (P = .08). Endocrine pancreas anatomy was not modified by the Western diet as compared to the normal diet (P = .93).
Conclusions
Early Western diet induced neuronal density and phenotype changes in PINS that might be involved in the pancreas insulin secretion dysfunctions associated with obesity.
Obesity starting in the young age has deep consequences on the maturation of the pancreas innervation (density and intrinsic nervous system phenotype) and the nervous control of insulin secretion. Western diet in childhood altered the normal course of pancreatic intrinsic nervous system maturation and impaired nicotinic control of insulin secretion.</description><subject>Diet</subject><subject>Digital cameras</subject><subject>endocrine pancreas</subject><subject>Endocrinology and metabolism</subject><subject>ex vivo model</subject><subject>Food and Nutrition</subject><subject>Glucose</subject><subject>Human health and pathology</subject><subject>Immunoglobulins</subject><subject>Insulin</subject><subject>Life Sciences</subject><subject>Motility</subject><subject>Nervous system</subject><subject>Obesity</subject><subject>Original</subject><subject>Pancreas</subject><subject>Western diet</subject><issn>2398-9238</issn><issn>2398-9238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp1ks1u1DAQgCMEolWpeANkiQNUaIt_EsfuAanaFlppERc4W44z2fUqsRc7WRROfQSekSfBIaVqK3GwbNmfv_GMJ8teEnxKMKbvoe7oqSyeZIeUSbGQlImn99YH2XGMW4wxkVJyTJ9nB4xISjGlh9nPCwv975tf1tWDgRr5CqLtR2QdGv3g1qizBs7Q0rsI3wdwBiLyDvUbQDvtTADdW5PoPlgX08pB2PshojjGHjpkfDrxLfJNYuLQJm2EdKu33r3InjW6jXB8Ox9l3z5efl1eLVZfPl0vz1cLk3NWLCoOsikNZqWuSiMbUeU1Y4XQREtJQUrDccF5CYUmdc4LDZzrPOe6JIQIQ9hRdj17a6-3ahdsp8OovLbq74YPa6VDyqIFxUVdUs0EwbTJBXCZgqQwhc6LpmJ1k1wfZtduqDqoDaT0dPtA-vDE2Y1a-73iMi_TSIKTWbB5dO3qfKWmPcw4kemr9tPD394GCz7VPvaqs9FA22oHqcaKMsZpLkrJEvr6Ebr1Q3CprBOVEyaEmKg3M2WCjzFAc_cCgtXUSmpqJSWLRL66n-cd969xEvBuBn7YFsb_edTlxWeadH8AFynTfQ</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Saade, Marie‐Béatrice</creator><creator>Cahu, Armelle</creator><creator>Moriez, Raphaël</creator><creator>Neunlist, Michel</creator><creator>Blat, Sophie</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2138-7078</orcidid><orcidid>https://orcid.org/0000-0003-1892-5071</orcidid></search><sort><creationdate>202001</creationdate><title>Diet‐induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion</title><author>Saade, Marie‐Béatrice ; Cahu, Armelle ; Moriez, Raphaël ; Neunlist, Michel ; Blat, Sophie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4635-b6e9f7c037ab7c9f8b4d3358a1a992e99c605667e5a1d465ae66a446a71118c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Diet</topic><topic>Digital cameras</topic><topic>endocrine pancreas</topic><topic>Endocrinology and metabolism</topic><topic>ex vivo model</topic><topic>Food and Nutrition</topic><topic>Glucose</topic><topic>Human health and pathology</topic><topic>Immunoglobulins</topic><topic>Insulin</topic><topic>Life Sciences</topic><topic>Motility</topic><topic>Nervous system</topic><topic>Obesity</topic><topic>Original</topic><topic>Pancreas</topic><topic>Western diet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saade, Marie‐Béatrice</creatorcontrib><creatorcontrib>Cahu, Armelle</creatorcontrib><creatorcontrib>Moriez, Raphaël</creatorcontrib><creatorcontrib>Neunlist, Michel</creatorcontrib><creatorcontrib>Blat, Sophie</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Open Access: DOAJ - Directory of Open Access Journals</collection><jtitle>Endocrinology, diabetes & metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saade, Marie‐Béatrice</au><au>Cahu, Armelle</au><au>Moriez, Raphaël</au><au>Neunlist, Michel</au><au>Blat, Sophie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diet‐induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion</atitle><jtitle>Endocrinology, diabetes & metabolism</jtitle><addtitle>Endocrinol Diabetes Metab</addtitle><date>2020-01</date><risdate>2020</risdate><volume>3</volume><issue>1</issue><spage>e00095</spage><epage>n/a</epage><pages>e00095-n/a</pages><issn>2398-9238</issn><eissn>2398-9238</eissn><abstract>Introduction
Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion.
Methods
Young mice (5‐week‐old, T0 group) were fed either a normal diet (ND group) or a Western diet (WD group) for 12 weeks. Pancreas nervous system density, PINS phenotype and pancreas anatomy were analysed by immunohistochemistry at T0 and in adulthood (ND and WD groups). Insulin secretion was also studied in these 3 groups using a new model of ex vivo pancreatic culture, where PINS was stimulated by nicotinic and nitrergic agonists with and without antagonists. Insulin was assayed in supernatants by ELISA.
Results
Pancreas nervous system density decreased with age in ND (P < .01) but not in WD mice (P = .08). Western diet decreased the PINS nitrergic component as compared to normal diet (P < .01) but it did not modify its cholinergic component (P = .50). Nicotinic PINS stimulation induced greater insulin secretion in ND compared to WD mice (P < .001) whereas nitrergic stimulation significantly decreased insulin secretion in ND mice (P < .001) and tended to increase insulin secretion in WD mice (P = .08). Endocrine pancreas anatomy was not modified by the Western diet as compared to the normal diet (P = .93).
Conclusions
Early Western diet induced neuronal density and phenotype changes in PINS that might be involved in the pancreas insulin secretion dysfunctions associated with obesity.
Obesity starting in the young age has deep consequences on the maturation of the pancreas innervation (density and intrinsic nervous system phenotype) and the nervous control of insulin secretion. Western diet in childhood altered the normal course of pancreatic intrinsic nervous system maturation and impaired nicotinic control of insulin secretion.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>31922022</pmid><doi>10.1002/edm2.95</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2138-7078</orcidid><orcidid>https://orcid.org/0000-0003-1892-5071</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Diet Digital cameras endocrine pancreas Endocrinology and metabolism ex vivo model Food and Nutrition Glucose Human health and pathology Immunoglobulins Insulin Life Sciences Motility Nervous system Obesity Original Pancreas Western diet |
| title | Diet‐induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion |
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