Approach of Supercritical Carbon Dioxide for the Extraction of Kleeb Bua Daeng Formula

Supercritical fluid extraction (SFE) is an innovative green technology for the extraction of phytochemicals from plants. Therefore, this study aimed to evaluate the application of SFE and to optimize the extraction conditions of the Thai herbal formula, Kleeb Bua Daeng (KBD). A Box–Behnken design (B...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-09, Vol.28 (19), p.6873
Main Authors: Ngamkhae, Nittaya, Monthakantirat, Orawan, Chulikhit, Yaowared, Maneenet, Juthamart, Khamphukdee, Charinya, Chotritthirong, Yutthana, Limsakul, Suphatson, Boonyarat, Chantana, Pitiporn, Supaporn, Kwankhao, Pakakrong, Kijjoa, Anake, Daodee, Supawadee
Format: Article
Language:English
Subjects:
Antioxidants
Box–Behnken design
Cancer
Carbon dioxide
Carotenoids
Cognitive ability
Flavonoids
Fruits
Isoflavones
Kleeb Bua Daeng formula
Medicine, Botanic
Medicine, Herbal
Optimization
Polyphenols
Product development
response surface methodology
Solvents
supercritical fluid extraction
Variables
Variance analysis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Supercritical fluid extraction (SFE) is an innovative green technology for the extraction of phytochemicals from plants. Therefore, this study aimed to evaluate the application of SFE and to optimize the extraction conditions of the Thai herbal formula, Kleeb Bua Daeng (KBD). A Box–Behnken design (BBD) with response surface methodology (RMS) was used to determine the effect of the extraction time (30–90 min), temperature (30–60 °C), and pressure (200–300 bar) on response variables including the extraction yield, total phenolic content (TPC), total flavonoid content (TFC), total carotenoid content (TCC), and total anthocyanin content (TAC) of the KBD formula. The highest percentage extraction yield (3.81%) was achieved at 60 °C, 300 bar, and 60 min of the extraction time. The highest TPC (464.56 mg gallic acid equivalents/g extract), TFC (217.19 mg quercetin equivalents/g extract), and TCC (22.26 mg β-carotene equivalents/g extract) were all achieved at 60 °C, 250 bar, and 90 min of the extraction time. On the contrary, it was not possible to quantify the total anthocyanin content as anthocyanins were not extracted by this method. The results indicated that SFE-CO2 is a suitable method of extraction for a green recovery of phytochemicals with low and moderate polarity from the KBD formula.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28196873