High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil

Hepatitis C virus (HCV) isolates have been divided into six genotypes (1 to 6). The duration of hepatitis C standard treatment is 48 weeks for patients infected with HCV genotype 1 vs 24 weeks for those infected with genotypes 2 and 3. A total of 1544 HCV isolates from chronic patients living in the...

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Published in:Memórias do Instituto Oswaldo Cruz 2007-11, Vol.102 (7), p.867-870
Main Authors: Silva, Cláudia Maria Dornelles da, Costi, Cintia, Krug, Luciano Percival, Ramos, Ana Beatris, Grandi, Tarciana, Gandolfi, Vitório Luiz, Menezes, Maria Elizabeth, Ocampos, Maristela, Niel, Christian, Rossetti, Maria Lucia Rosa
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Brazil
Cohort Studies
Female
Genotype
Hepacivirus - genetics
Hepatitis C virus
hepatitis C virus genotypes
Hepatitis C, Chronic - virology
Humans
Male
Middle Aged
nucleotide sequencing
PARASITOLOGY
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
restriction fragment lengh polymorphism
Retrospective Studies
Rio Grande do Sul
RNA, Viral - genetics
Santa Catarina
TROPICAL MEDICINE
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Summary:Hepatitis C virus (HCV) isolates have been divided into six genotypes (1 to 6). The duration of hepatitis C standard treatment is 48 weeks for patients infected with HCV genotype 1 vs 24 weeks for those infected with genotypes 2 and 3. A total of 1544 HCV isolates from chronic patients living in the southern Brazilian states of Rio Grande do Sul (RS, n=627) and Santa Catarina (SC, n=917) were genotyped by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. In RS, 338 (53.9%; 95% CI 50.0-57.8%), 34 (5.4%; 95% CI 3.8-7.4%) and, 255 (40.7%; 95% CI 36.9-44.6%) samples were from genotypes 1, 2, and 3, respectively. In SC, 468 (51%; 95% CI 47.8-54.2%), 26 (2.9%; 95% CI 1.9-4.1%) and, 423 (46.1%; 95% CI 42.9-49.3%) samples were from genotypes 1, 2, and 3, respectively. Genotyping results were confirmed by direct nucleotide sequencing of PCR products derived from 68 samples, without any discrepancy between PCR-RFLP and nucleotide sequencing methods. In conclusion, almost half of the hepatitis C patients from South of Brazil are infected by genotypes 2 and 3 and, these results have important consequential therapeutic implications as they can be treated for only 24 weeks, not 48.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/s0074-02762007005000122