Investigation in vivo of Enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods

This study aimed to investigate the prevalence of E. faecalis in root-filled canals using culture and molecular approaches. It was evaluated the antimicrobial susceptibility to different antibiotics and the virulence factors of E. faecalis isolates. Microbial samples were taken from thirty root-fill...

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Bibliographic Details
Published in:Acta scientiarum. Health sciences 2015-01, Vol.37 (1), p.95-103
Main Authors: Endo, Marcos Sergio, Signoretti, Fernanda Graziela Corrêa, Kitayama, Vivian Sayuri, Marinho, Ariane Cássia Salustiano, Martinho, Frederico Canato, Gomes, Brenda Brenda Paula F. de Almeida
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
antimicrobial susceptibility
Dentistry
endodontics
Studies
virulence
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Summary:This study aimed to investigate the prevalence of E. faecalis in root-filled canals using culture and molecular approaches. It was evaluated the antimicrobial susceptibility to different antibiotics and the virulence factors of E. faecalis isolates. Microbial samples were taken from thirty root-filled canals. Culture methods and 16S rDNA assay were used to identify E. faecalis. The antimicrobial susceptibility of E. faecalis was determined by MIC values using the E test. Cultivable strains of E. faecalis were investigated for virulence factors by PCR technique. E. faecalis were detected by culture (7/30), traditional PCR assay (13/30) and nested PCR (23/30). Both PCR were significantly more effective than culture in detecting E. faecalis (p < 0.05). All tested E. faecalis were highly sensitive to amoxicillin. Some strains of E. faecalis were resistant to antibiotics such as rifampicin (4/12), erythromycin (3/12) and azythromycin (8/12). The genes efaA and ace were detected in all isolates. The other virulence genes were found in 91.6 (gelE), 83.3 (asa), 25 (esp) and 16.6% (cylA). Strains of E. faecalis isolated from root-filled canals showed virulence factors related to adherence. They also showed resistance to some antibiotics commonly used in dentistry.
ISSN:1679-9291
1807-8648
DOI:10.4025/actascihealthsci.v37i1.24348