TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

PURPOSE: To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS: We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture m...

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Bibliographic Details
Published in:Acta cirúrgica brasileira 2014, Vol.29 (suppl 1), p.1-6
Main Authors: Aloise, Antonio Carlos, Pereira, Max Domingues, Duailibi, Silvio Eduardo, Gragnani, Alfredo, Ferreira, Lydia Masako
Format: Article
Language:English
Subjects:
Cell differentiation
Dexamethasone
Fibroblasts
Transforming Growth Factor beta1
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Summary:PURPOSE: To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS: We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 mmol/l of β-glycerophosphate and 10 nmol/L of dexamethasone); and OSTEOG/TGF-β1 (osteogenic medium with 10 ng/ml of TGF-β1). Alkaline phosphatase (ALP) activity and the amount of osteocalcin (OC) in the supernatant, as well as the capability to form calcium phosphate deposits, were analysed for 28 days RESULTS: There were significant differences (p
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-86502014001300001