Bio-fabrication of Zinc Oxide nanoparticles to rescue Mung Bean against Cercospora leaf spot disease

• Published in: Frontiers in plant science 2022-11, Vol.13, p.1052984-1052984
• Main Authors: Rafiq, Hamza, Aftab, Zill-E-Huma, Anjum, Tehmina, Ali, Basharat, Akram, Waheed, Bashir, Uzma, Mirza, Faisal Shafiq, Aftab, Muzammil, Ali, Muhammad Danish, Li, Guihua
• Format: Article
• Language: English
• Subjects: antifungal; cercospora leaf spot; green synthesis; Plant Science; trachyspermum ammi; zinc oxide nanoparticles
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.1052984

Plant disease management using nanotechnology is evolving continuously across the world. The purpose of this study was to determine the effect of different concentrations of green synthesized zinc oxide nanoparticles (ZnO NPs) using seed extract on Cercospora leaf spot disease in mung bean plants under and conditions. Additionally, the effects on mung bean agronomic and physiological parameters were also assessed. The green synthesized ZnO NPs were characterized using UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Scanning electron microscopy (SEM). Green synthesized NPs were tested for their ability to inhibit fungal growth at five different concentrations under experiment. After 7 days of inoculation, ZnO NPs (1200 ppm) inhibited mycelial growth substantially (89.86% ± 0.70). The experiment showed statistically significant result of disease control (30% ± 11.54) in response to 1200 ppm ZnO NPs. The same treatment showed statistically significant improvements in shoot length, root length, number of leaves, number of pods, shoot fresh weight (28.62%), shoot dry weight (85.18%), root fresh weight (38.88%), and root dry weight (38.88%) compared to the control. Our findings show that green synthesized ZnO NPs can control in mung bean, pointing to their use in plant disease control and growth enhancement.