Argyrophilic nucleolar organizer regions in inflammatory, premalignant, and malignant oral lesions: a quantitative and qualitative assessment

• Published in: Indian journal of dental research 2008-04, Vol.19 (2), p.141-146
• Main Authors: Elangovan, T, Mani, N J, Malathi, N
• Format: Article
• Language: English
• Subjects: AgNORS; Antigens, Nuclear - analysis; Biomarkers, Tumor; Carcinoma, Squamous Cell - chemistry; Carcinoma, Squamous Cell - pathology; Cell Proliferation; Dentistry; Diagnosis; Diagnosis, Differential; Granuloma, Pyogenic - pathology; Health aspects; Humans; Leukoplakia, Oral - chemistry; Leukoplakia, Oral - pathology; Mouth Diseases - pathology; Mouth Mucosa - chemistry; Mouth Mucosa - cytology; Mouth Neoplasms - chemistry; Mouth Neoplasms - pathology; nucleolar organizer regions; Nucleolus organizer region; Nucleolus Organizer Region - pathology; oral cancer; oral lesions; Physiological aspects; Precancerous Conditions - chemistry; Precancerous Conditions - pathology; premalignant lesions/conditions; Proteins; qualitative and quantitative assessment; Silver Staining; Studies; Tumors
• ISSN: 0970-9290; 1998-3603
• DOI: 10.4103/0970-9290.40469

Argyrophilic nucleolar organizer regions (AgNORs) have found widespread application in the past, especially in tumor histopathology. This study was undertaken to evaluate the significance of various AgNOR parameters and to assess their role in differentiating hyperplastic, premalignant, and malignant lesions. The study sample consisted of archival biopsy specimens of ten squamous cell carcinomas, ten premalignant lesions, and five inflammatory lesions. Two biopsies from normal mucosa acted as control. AgNORs were assessed both quantitatively and qualitatively. The data were analyzed using Student's independent t-test, one-way analysis of variance (ANOVA), and multiple range test (Tukey-HSD). Quantitatively significant difference existed in the number of AgNORs between the normal mucosa, inflammatory lesions, and carcinomas, but the premalignant lesions failed to differ significantly from the normal mucosa. The number of AgNORs was found to be related to epithelial proliferation. Qualitatively, in terms of size, shape, and pattern of distribution, the normal mucosa and inflammatory lesion were alike, but the premalignant and malignant lesions differed significantly from the normal, with a marked degree of AgNOR pleomorphism being observed in carcinomas. AgNOR quantity is strictly proportional to the proliferative activity of the cell and does not necessarily indicate malignancy. It is the qualitative characteristics of AgNOR that help to differentiate hyperplastic, premalignant, and malignant lesions.