Arabidopsis G-Protein β Subunit AGB1 Negatively Regulates DNA Binding of MYB62, a Suppressor in the Gibberellin Pathway

Plant G proteins are versatile components of transmembrane signaling transduction pathways. The deficient mutant of heterotrimeric G protein leads to defects in plant growth and development, suggesting that it regulates the GA pathway in Arabidopsis. However, the molecular mechanism of G protein reg...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (15), p.8270
Main Authors: Qi, Xin, Tang, Wensi, Li, Weiwei, He, Zhang, Xu, Weiya, Fan, Zhijin, Zhou, Yongbin, Wang, Chunxiao, Xu, Zhaoshi, Chen, Jun, Gao, Shiqin, Ma, Youzhi, Chen, Ming
Format: Article
Language:English
Subjects:
AGB1
Arabidopsis
Binding
Cell division
Deficient mutant
Deoxyribonucleic acid
DNA
GA signaling
Genetic analysis
Gibberellins
Kinases
Morphology
MYB62
protein interaction
Proteins
Seeds
Signal transduction
Transcription
Transcription factors
Transgenic plants
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Summary:Plant G proteins are versatile components of transmembrane signaling transduction pathways. The deficient mutant of heterotrimeric G protein leads to defects in plant growth and development, suggesting that it regulates the GA pathway in Arabidopsis. However, the molecular mechanism of G protein regulation of the GA pathway is not understood in plants. In this study, two G protein β subunit (AGB1) mutants, agb1-2 and N692967, were dwarfed after exogenous application of GA3. AGB1 interacts with the DNA-binding domain MYB62, a GA pathway suppressor. Transgenic plants were obtained through overexpression of MYB62 in two backgrounds including the wild-type (MYB62/WT Col-0) and agb1 mutants (MYB62/agb1) in Arabidopsis. Genetic analysis showed that under GA3 treatment, the height of the transgenic plants MYB62/WT and MYB62/agb1 was lower than that of WT. The height of MYB62/agb1 plants was closer to MYB62/WT plants and higher than that of mutants agb1-2 and N692967, suggesting that MYB62 is downstream of AGB1 in the GA pathway. qRT-PCR and competitive DNA binding assays indicated that MYB62 can bind MYB elements in the promoter of GA2ox7, a GA degradation gene, to activate GA2ox7 transcription. AGB1 affected binding of MYB62 on the promoter of GA2ox7, thereby negatively regulating th eactivity of MYB62.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22158270