Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots

Boron (B) is a micronutrient for plant development, and its deficiency alters many physiological processes. However, the current knowledge on how plants are able to sense the B-starvation signal is still very limited. Recently, it has been reported that B deprivation induces an increase in cytosolic...

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Published in:International journal of molecular sciences 2019-05, Vol.20 (9), p.2297
Main Authors: Quiles-Pando, Carlos, Navarro-Gochicoa, M. Teresa, Herrera-Rodríguez, M. Begoña, Camacho-Cristóbal, Juan J., González-Fontes, Agustín, Rexach, Jesús
Format: Article
Language:English
Subjects:
Abscisic acid
Apoplast
apoplastic calcium
Arabidopsis thaliana
Boron
boron deficiency
Calcium influx
Calcium ions
calcium signaling
Cameleon YC3.6
Cell walls
Changing environments
Cytoskeleton
cytosolic calcium
Deprivation
Endoplasmic reticulum
Flowers & plants
Homeostasis
Kinases
Nutrient availability
Nutrients
Oxidative metabolism
Oxidative stress
Plants
Rhamnogalacturonan
Seedlings
Structure-function relationships
Sulfates
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Summary:Boron (B) is a micronutrient for plant development, and its deficiency alters many physiological processes. However, the current knowledge on how plants are able to sense the B-starvation signal is still very limited. Recently, it has been reported that B deprivation induces an increase in cytosolic calcium concentration ([Ca2+]cyt) in Arabidopsis thaliana roots. The aim of this work was to research in Arabidopsis whether [Ca2+]cyt is restored to initial levels when B is resupplied and elucidate whether apoplastic Ca2+ is the major source for B-deficiency-induced rise in [Ca2+]cyt. The use of chemical compounds affecting Ca2+ homeostasis showed that the rise in root [Ca2+]cyt induced by B deficiency was predominantly owed to Ca2+ influx from the apoplast through plasma membrane Ca2+ channels in an IP3-independent manner. Furthermore, B resupply restored the root [Ca2+]cyt. Interestingly, expression levels of genes encoding Ca2+ transporters (ACA10, plasma membrane PIIB-type Ca2+-ATPase; and CAX3, vacuolar cation/proton exchanger) were upregulated by ethylene glycol tetraacetic acid (EGTA) and abscisic acid (ABA). The results pointed out that ACA10, and especially CAX3, would play a major role in the restoration of Ca2+ homeostasis after 24 h of B deficiency.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20092297