Synthesis, anticancer evaluation, and molecular modeling study of new 2-(phenylamino)pyrazolo[1,5-a]pyrimidine analogues

• Published in: Arabian journal of chemistry 2023-01, Vol.16 (1), p.104437, Article 104437
• Main Authors: Azher, Omer A., Hossan, Aisha, Pashameah, Rami A., Alsoliemy, Amerah, Alharbi, Arwa, Habeebullah, Turki M., El-Metwaly, Nashwa M.
• Format: Article
• Language: English
• Subjects: 3-aminopyrazoles; MCF-7; Molecular docking; Molecular modelling; Pyrazolopyrimidines
• ISSN: 1878-5352; 1878-5379
• DOI: 10.1016/j.arabjc.2022.104437

The reaction of 3-amino-5-phenylaminopyrazoles 2 with 3-(dimethylamino) acrylonitrile derivatives resulted in a series of substituted pyrazolopyrimidine analogues 4 and 6. The DFT studies of the isolated compounds showed that the frontier molecular orbitals energy gap was close and in the 2.65–2.81 eV range where the derivative 6b has the lowest and both of 4a and 4c have the highest values. Meanwhile, the anticancer activity of the newly synthesized pyrazolopyrimidine analogues have been tested against several different cell lines (MCF-7, PC3, Hep-2 and WI38). The investigated pyrazolopyrimidines showed remarkable cytotoxicity activity against the MCF-7 and Hep-2 cell lines. In comparison to the effects of 5-fluorouracil, IC50 = 10.19 ± 0.42 and 7.19 ± 0.47, compounds 6a-c demonstrated potential anticancer activity with IC50 values for MCF-7 (10.80 ± 0.36–19.84 ± 0.49 μM) and Hep-2 (8.85 ± 0.24–12.76 ± 0.16 μM). Important details regarding the protein's binding sites were disclosed when the produced analogues docked with the crystal structure of the KDM5A protein, which was located in the protein data library.