Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits

• Published in: Journal of global antimicrobial resistance. 2023-06, Vol.33, p.78-82
• Main Authors: Islam, Md. Maidul, Kim, Jeongah, Kim, Kyeongmin, Kim, Dooyoung, Akter, Samia, Bang, Seunghyeok, Kim, Shukho, Kim, Jungmin, Lee, Je Chul, Shin, Minsang
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii KBN10P05679; Antibiotic resistance; Biofilm formation; Virulence; Whole-genome sequencing (WGS)
• ISSN: 2213-7165; 2213-7173
• DOI: 10.1016/j.jgar.2023.02.016

•Acinetobacter baumannii exhibits antimicrobial resistance to several drugs.•KBN10P05679 harbors genes for antibiotic resistance and virulence factors.•KBN10P05679 is resistant to all tested antibiotics except colistin.•KBN10P05679 has higher biofilm-forming ability than standard A. baumannii strains.•New strategies are needed for effective treatment of A. baumannii infections. Acinetobacter baumannii, a nosocomial pathogen, exhibits multidrug resistance and is a major concern worldwide. We therefore aimed to evaluate the genomic features of the clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence factors. In silico multilocus sequence typing, phylogenetic identification, genome annotation, genome analysis, antibiotic susceptibility testing, and biofilm formation assay were performed, and the expression levels of antibiotic resistance- and biofilm-related genes were investigated. The complete genome of KBN10P05679 comprises a circular chromosome of 3 990 428 bp and two plasmids (74 294 and 8731 bp) and was assigned to the ST451 sequence type. Clusters of Orthologous Gene annotation identified 3810 genes, including those involved in amino acid transport and metabolism, transcription, inorganic ion transport, energy production and conversion, replication, recombination and repair, and carbohydrate and protein metabolism. The antibiotic resistance genes were investigated by searching the Comprehensive Antibiotic Resistance Database, and the genome was found to harbour 30 different antibiotic resistance genes. Analysis of the Virulence Factor Database revealed 86 virulence factor genes in the KBN1005679 genome. The KBN10P05679 strain was found to have a higher capacity for biofilm formation and expressed biofilm-related genes at a higher level than the other tested strains. The antibiotic resistance genotype and potential virulence factor-related data obtained in this study would help direct future studies for developing the control measures for this multidrug-resistant pathogen.